Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome of potato responding to Ralstonia solanacearum

ABSTRACT: Proteomic analysis of potato responding to the invasion of Ralstonia solanacearum UW551 and its type III secretion system mutant

ORGANISM(S): Solanum Tuberosum Ralstonia Solanacearum Po82

SUBMITTER: Huilan Chen

PROVIDER: PXD021355 | iProX | Tue Sep 08 00:00:00 BST 2020

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Proteomic Analysis of Potato Responding to the Invasion of Ralstonia solanacearum UW551 and Its Type III Secretion System Mutant.

Wang Bingsen B He Tianjiu T Zheng Xueao X Song Botao B Chen Huilan H

Molecular plant-microbe interactions : MPMI 20210324 4

The infection of potato with Ralstonia solanacearum UW551 gives rise to bacterial wilt disease via colonization of roots. The type III secretion system (T3SS) is a determinant factor for the pathogenicity of R. solanacearum. To fully understand perturbations in potato by R. solanacearum type III effectors(T3Es), we used proteomics to measure differences in potato root protein abundance after inoculation with R. solanacearum UW551 and the T3SS mutant (UW551△HrcV ...[more]

PMID: 33332146

Similar Datasets

The YfgL lipoprotein is required for cell adhesion and invasion, type I fimbriae expression and virulence of Klebsiella pneomoniae infecion

Project description:To address and clarify the regulatory function of YfgL in K. pneumoniae, the transcriptome analyses of the yfgL mutant and the wild-type strain were compared. K. pneumoniae strain deleted for yfgL exhibited reduced adherence and invasion to host cells. A previous study has demonstrated that the YfgL lipoprotein is essential for type III secretion system expression of Salmonella enterica serovar Enteritidis. Several putative secretion systems, but not type III secretion system, were already identified of the genome of the NTUH-K2044. Transcription of type 1 fimbriae was more highly induced in the wild-type than in the yfgL mutant strain. In addition to the fim gene cluster, genes encoding some amino acid metabolism, peptidoglycan synthesis and putative phosphoenopyruvate:carbohydrate phosphotransferase system (PTS), including the tdc gene cluster, yraM and the kp0760-kp0763 (pts) gene cluster, were also up-regulated by YfgL. Among a total 2,848 cloned sequences covering 99.9% of the entire genome sequence of NTUH-K2044, the expression of 9 cloned sequences was up-regulated and 3 cloned sequences were down-regulated at a cut-off value of 2.

2016-05-25 | GSE69327 | GEO

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920

Project description:Photoinduction of the Vibrio campbellii Type III Secretion System

| PRJNA152039 | ENA

Insight on genes affecting tuber development in potato upon Potato spindle tuber viroid (PSTVd) infection

Project description:Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, it can replicate in the nucleus and the viroid RNA moves systemically in infected plants. Its KF440-2 strain can cause severe symptoms in potato. It is not well understood how the viroid can affect host genes for successful invasion and which genes show altered expression levels upon infection. In this study, we used a high-scale method to identify differentially expressed genes in potato. We have identified defence, stress and sugar metabolism related genes having altered expression levels upon infection. Additionally, hormone pathways connected genes showed up- or down-regulation. Our primary focus is on the identification of genes which can affect tuber formation as the viroid infection can strongly influence tuber development, especially tuber shape is affected. DWARF1/DIMINUTO, Gibberellin 7-oxidase and BEL5 protein were identified and validated which showed differential expression in viroid infected tissues suggesting that gibberellin and brassinosteroid pathways have a possible role in tuber development upon PSTVd infection.

2016-02-01 | E-MTAB-3869 | biostudies-arrayexpress

Potato virus Y infection hinders potato defence response and renders plants more vulnerable to Colorado potato beetle attack

Project description:In the present study molecular interactions between potato plants, Colorado potato beetle (CPB) larvae and Potato virus YNTN (PVYNTN) were investigated by analyzing gene expression in potato leaves. Grant ID: J4-4165 Slovenian Research Agency ARRS Growth and defense trade-offs in multitrophic interaction between potato and its two major pests Grant ID: P4-0165 Slovenian Research Agency ARRS Biotechnology and Plant Systems Biology

2014-10-23 | GSE56333 | GEO

cDNA microarray data after influenza A virus infection in mouse lung tissue

Project description:The defense mechanisms that are provided by the innate immune system are a formidable barrier to influenza virus and a special immune system exists at distinct respiratory epithelium to combat invasion by influenza virus. Innate immune mechanisms for antiviral resistance are mediated by an increase of interferons’ secretion and type I and III IFNs represent the prototypical resistance mechanism as they induce diverse of Interferon stimulated genes that serve as effectors to limit viral replication.

2015-07-08 | GSE70646 | GEO

Gene regulation by the RcsCB two-component system and the auxiliary protein TviA in S. Typhi

Project description:In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasion associated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion and reduced expression of FliC. Both RcsB and TviA reduced expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity. Gene expression profiles of the S. Typhi wild-type strain, a rcsB mutant, a (delta)tviB-vexE (tviBCDEvexABCDE) mutant, and a (delta)viaB (tviABCDEvexABCDE) mutant (lacking tviA expression) was compared. Strains were cultured in SOB broth (low salt), a condition known to induce expression of TviA and the RcsCB system. Total RNA was extracted from two independent cultures (biological replicates) of each strain. cDNA was differentially labeled with Alexa Fluor 555 and 648, respectively and competitively hybridized on S.Typhimurium arrays (PFGRC, version 4). The submitted samples are biological replicates of a competitive hybridization of cDNA from the wild-type strain (Ty2) and the rcsB mutant (SW237) (GSM395580, GSM395581) or the viaB mutant (STY2) and the tviB-vexE mutant (SW74) (GSM395582, GSM395583), respectively.

2010-05-18 | E-GEOD-15752 | biostudies-arrayexpress

Lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium Dickeya solani.

Project description:The Lon protein is a protease implicated in virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria include important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of MS-SWATCH analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant.

2021-09-09 | PXD018297 | Pride

A transcriptome study of DSF-mediated quorum sensing in Xanthomonas axonopodis pv. citri

Project description:Quorum sensing (QS) in Xanthomonas axonopodis pv. citri, the causal agent of citrus canker, is mediated by a diffusible signal factor (DSF). QS is required for the full virulence of X. axonopodis pv. citri in planta. Mutations in rpfF, rpfC and rpfG, the core genes of QS, decreased the production of extracellular proteases and bacterial motility. Comparison of the transcriptomes of QS mutants with that of the wild type stain revealed that QS temporally regulates the expression of a large set of genes, including genes involved in chemotaxis and flagellar biosynthesis, genes related to metabolism, genes encoding virulence traits such as type II secretion system substates, type III secretion system and effectors. Cross talk between the QS regulon and the HrpG regulon has also been identified by 62 common genes shared by both regulons. The temporal regulation of the QS regulon and cross talk with the HrpG regulon suggest the important role of QS in citrus canker infection, including attachment, invasion and growth in host apoplast. Three mutants and two time-points experiment. Four biological and dye-swap replicates of each strain per each time-point were used. In total, there were two datasets of the rpfF mutant: 1) rpfF mutant vs. wild type strain at 11 h, and 2) rpfF mutant vs. wild type strain at 25 h; two datasets of the rpfC mutant: 1) rpfC mutant vs. wild type strain at 11 h, and 2) rpfC mutant vs. wild type strain at 25 h; and two datasets of the rpfG mutant: 1) rpfG mutant vs. wild type strain at 11 h, and 2) rpfG mutant vs. wild type strain at 25 h.

2012-04-26 | E-GEOD-29877 | biostudies-arrayexpress

Comprehensive Transcriptome and Proteome Analyses in Potato Roots

Project description:The root system plays a vital role in the growth and development of plants. The water requirements of the root system depend largely on the species. As a globally important food and vegetable crop, potato is mainly grown in arid and semi-arid regions; however, global warming is expected to have a great impact on potato yields, so it is important to study the genes related to root development and drought-resistant signaling pathways in potato.

2023-10-31 | PXD046520 | iProX

RNA-seq reveals quorum sensing-dependent activation of genes involved in ecological fitness during Pcb1692 potato-host infection

Project description:Pectobacterium carotovorum subsp. brasiliense 1692 (Pcb1692) is an emerging pathogen of significance affecting potato production globally. In the current study, we reveal the complete transcriptome of Pcb in planta at the early and later stages of infection (24 and 72 hour post inoculation (hpi), respectively) using RNA-seq. We identified713 differentially expressed genes at 24hpi and an additional 157 differentially expressed genes at 72 hpi. Genes encoding PCWDEs and iron acquisition were up-regulated. The type VI secretion system (T6SS) and the bacteriocin (carotovoricin) gene clusters were both highly up-regulated, suggesting they might play a role in antibacterial activity in eliminating competition for nutrients with other microbes within the niche. Additionally, our results suggest the importance of genes involved in amino acid, carbohydrates, citrate metabolism, adhesion, chemotaxis, motility, metabolism as these were differentially expressed at 24 hpi. To establish the Pcb1692 quorum sensing regulon, the transcriptome of Pcb1692 wild-type compared to that of Pcb1692∆expI during in vitro growth and in planta at 24 hpi in potato tubers was analyzed. Important virulence factors such as T2SS, T6SS, and citrate metabolism were found to be under quorum sensing regulation while, carotovoricin did not appear to be quorum sensing dependent.

2018-09-01 | GSE102557 | GEO