Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of the methyl-CpG binding protein 2 (MeCP2) interacting proteins in the high molecular weight (HMW) extract prepared from the human HEK293T cells by immunoprecipitation coupled with mass spectrometry analysis

ABSTRACT: Identification of MeCP2 interacting proteins in HMW extract of HEK293T cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Jingyi Hui

PROVIDER: PXD021650 | iProX | Mon Aug 30 00:00:00 BST 2021

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Rett syndrome linked to defects in forming the MeCP2/Rbfox/LASR complex in mouse models.

Jiang Yan Y Fu Xing X Zhang Yuhan Y Wang Shen-Fei SF Zhu Hong H Wang Wei-Kang WK Zhang Lin L Wu Ping P Wong Catherine C L CCL Li Jinsong J Ma Jinbiao J Guan Ji-Song JS Huang Ying Y Hui Jingyi J

Nature communications 20211001 1

Rett syndrome (RTT) is a severe neurological disorder and a leading cause of intellectual disability in young females. RTT is mainly caused by mutations found in the X-linked gene encoding methyl-CpG binding protein 2 (MeCP2). Despite extensive studies, the molecular mechanism underlying RTT pathogenesis is still poorly understood. Here, we report MeCP2 as a key subunit of a higher-order multiunit protein complex Rbfox/LASR. Defective MeCP2 in RTT mouse models disrupts the assembly of the MeCP2/ ...[more]

PMID: 34599184

Similar Datasets

Identification of the Zbtb46 interacting proteins in the extract prepared from Zbtb46-Flag transfected HEK293T cells by IP coupled with mass spectrometry analysis

Project description:Identification of the zbtb46 interacting proteins in the extract prepared from zbtb46-flag transfected HEK293T cells

2021-09-01 | PXD028195 |

Identification of high molecular weight protein/protein complexes in human cultured cells (HEK293T) LC-MS/MS

Project description:Phosphoribosyl pyrophosphate synthetase (PRPS) is a highly conserved enzyme that conducts the chokepoint reaction of nucleotide biosynthesis by converting ribose-5-phosphate (R-5-P) to phosphoribosyl pyrophosphate (PRPP). Due to the presence of multiple isoforms and related PRPS-associated proteins in opisthokont species, the precise nature of the PRPS enzyme in cells and tissues remains uncertain. Using proteomics approaches and biochemistry, we demonstrate that these individual components assemble together to form a heterogeneous megadalton complex comprising of PRPS1, PRPS2, and two PRPS associated proteins – PRPSAP1 and PRPSAP2. To validate this and identify other protein/protein complexes that exists in a similar high molecular weight (HMW) range in human (HEK293T) cells, we performed shotgun proteomics on size exclusion chromatography fractions containing HMW proteins/protein complexes. This analysis identified a total of 262 unique proteins, which included ribosomal proteins and CAD confirming enrichment for HMW proteins. This dataset also revealed that among the eight cytosolic enzymes residing in HMW range, two were PRPS isozymes, indicating that the PRPS

2025-05-13 | PXD059156 | Pride

Human HEK293T nuclear extract LC-MSMS

Project description:Five tandem-domain reader proteins (BPTF, CBP, TAF1, TRIM24 and TRIM33) were designed and prepared as the enriched probes, and a group of binding proteins from HEK293T were enriched by domain probes and identified by HPLC-MS/MS.

2020-01-13 | PXD014909 | Pride

Multicomponent Oleogels Prepared with High- and Low-Molecular-Weight Oleogelators: Ethylcellulose and Waxes.

Project description:The combined interactions between ethylcellulose (EC) and natural waxes to structure edible oil are underexplored. To reduce the high EC concentration required to form a functional oleogel, novel oleogels were prepared using a 50% critical concentration of EC (i.e., 4%) with 1-4% beeswax (BW) and carnauba wax (CRW). One percent wax was sufficient for EC to form self-sustaining oleogel. Rheological analysis demonstrated that 4%EC + 4%BW/CRW had comparable oleogel properties to 8%EC. The yield stress and flow point of wax oleogels were enhanced upon EC addition. EC did not influence the thermal behaviour of the wax component of the oleogel, but the crystallinity and plasticity of the combined oleogel increased. The crystal shape of BW oleogel changed upon EC addition from a needle-like to spherulitic shape. Confocal laser scanning microscopy highlighted the uniform distribution of EC polymeric network and wax crystals. EC/wax mixtures have promising oil-structuring abilities that have the potential to use as solid fat substitutes.

| S-EPMC10453496 | biostudies-literature

HEK293T 15xGly/99xGly immunoprecipitation-mass spectrometry

Project description:In order to determine the protein composition of polyglycine aggregates, clonal HEK293T cell lines harboring doxycycline (DOX)-suppressible 15xGly-eGFP-3xHA or 99xGly-eGFP-3xHA constructs were grown either in standard media for 14 days (induction of transgene) or maintained in DOX (suppression of transgene).

2025-07-18 | PXD046400 | Pride

HEK293T cells

Project description:HEK293T cells Raw sequence reads

| PRJNA905048 | ENA

HEK293T cells

Project description:HEK293T cells Raw sequence reads

| PRJNA948481 | ENA

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells Results: Differences in a set of microRNAs Conclusions: Nef-transfection induces changes in the microRNA content of exosomes

2018-11-18 | GSE122656 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells. Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells. Results: Differences in a set of mRNAs. Conclusions: Nef-transfection might induces changes in the mRNA content of exosomes.

2018-11-18 | GSE122655 | GEO

Optimizing high molecular weight extractions

Project description:Optimizing high molecular weight extractions

| PRJNA1114081 | ENA