Proteomics

Dataset Information

0

HBXIP-mediated cell cycle, apoptosis and autophagy participate in the inhibitory effect of Germacrone on gastric cancer cells

ABSTRACT: As a monocyclic sesquiterpene, Germacrone has remarkable anti-tumor effect in a variety of cancers such as hepatocellular carcinoma, gastric cancer, and breast cancer. However, the mechanism of Germacrone on gastric cancer was still unclear. In our study, Germacrone inhibited gastric cancer cells proliferation in a dose-dependent manner. As shown, Germacrone induced G0 / G1 phase arrest and apoptosis in gastric cancer. Germacrone increased the expression of LC3ii/LC3i, p62. Then combined with CQ, the expression of LC3ii/LC3i, p62 continued to increase. As a result, Germacrone partially blocked the autophagy process. Through proteomic technology, a total of 596 proteins were screened and the top hits were Hepatitis B X-interacting protein (HBXIP). Overexpression of HBXIP delayed the cycle arrest, induction of apoptosis, and autophagy inhibition induced by Germacrone. Germacrone inhibits proliferation in gastric cancer cells by inhibiting HBXIP, and this process is related to G0 / G1 phase arrest, induction apoptosis, and autophagy inhibition.

ORGANISM(S): Homo Sapiens

SUBMITTER: Quan Xia  

PROVIDER: PXD021980 | iProX | Wed Oct 14 00:00:00 BST 2020

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells.

Fang Xing X   Tan TingFei T   Gao BeiBei B   Zhao YingLi Y   Liu TingTing T   Xia Quan Q  

Frontiers in oncology 20201110

Germacrone, a monocyclic sesquiterpene, exerts marked antitumor effects in a variety of cancers, including hepatocellular carcinoma, gastric cancer, and breast cancer. However, the mechanism underlying the effects of germacrone on gastric cancer remains unclear. In this study, we show that germacrone inhibited gastric cancer cell proliferation in a dose-dependent manner, and induced G0/G1-phase cell cycle arrest and apoptosis in these cells. Moreover, germacrone increased the expression of LC3II  ...[more]

Similar Datasets

Transcriptomics MET Amplified Gastric Cancer Cell Lines +/- Crizotinib
2016-05-13 | E-GEOD-77320 | biostudies-arrayexpress
Transcriptomics Prolonged halting of gene expression in early G1 arrest by inhibition of CDK4/CDK6 reprograms myeloma cells for cytotoxic killing
2012-04-06 | E-GEOD-35728 | biostudies-arrayexpress
Transcriptomics Prolonged halting of gene expression in early G1 arrest by inhibition of CDK4/CDK6 reprograms myeloma cells for cytotoxic killing
2012-04-07 | GSE35728 | GEO
Transcriptomics Gene expression changes initiated by miR-34c in Dicer/Pten double knockout mouse serous epithelial cancer
2014-10-03 | GSE57493 | GEO
Transcriptomics Gene expression changes initiated by miR-34c in Dicer/Pten double knockout mouse serous epithelial cancer
2014-10-03 | E-GEOD-57493 | biostudies-arrayexpress
Proteomics CDK4/6 inhibitor-mediated cell overgrowth triggers an osmotic stress response
2023-11-27 | PXD036519 | Pride
Proteomics Oncogenic signals prime cancer cells for toxic cell growth during a G1 cell cycle arrest
2023-11-17 | PXD043792 | Pride
Transcriptomics Long non-coding RNA ncRuPAR regulates gastric cancer cell proliferation and apoptosis via phosphoinositide 3-kinase/protein kinase B signaling
2022-08-19 | GSE211598 | GEO
Metabolomics,Multiomics Maslinic acid-enriched diet decreases intestinal tumorigenesis in Apc(Min/+) mice through transcriptomic and metabolomic reprogramming
2017-02-09 | MTBLS240 | MetaboLights
Proteomics CDK4/6 inhibitors induce repication stress to cause long-term cell cycle withdrawal
2021-12-20 | PXD023435 | Pride