Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Prefrontal cortex proteomics of CMS-treated rats

ABSTRACT: We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the prefrontal cortex were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Jian Zhou

PROVIDER: PXD022045 | iProX | Fri Oct 16 00:00:00 BST 2020

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Chronic mild stress-induced protein dysregulations correlated with susceptibility and resiliency to depression or anxiety revealed by quantitative proteomics of the rat prefrontal cortex.

Liao Wei W Liu Yanchen Y Wang Lixiang L Cai Xiao X Xie Hong H Yi Faping F Huang Rongzhong R Fang Chui C Xie Peng P Zhou Jian J

Translational psychiatry 20210224 1

Chronic stress is a significant risk factor for depression as well as anxiety disorders. Yet, the stress-induced specific and common molecular dysregulations of these disorders have not been fully understood. Previously, we constructed a chronic mild stress (CMS) rat model to separate and obtain depression-susceptible, anxiety-susceptible, and insusceptible groups. In this study, the prefrontal cortical proteomes of the three stressed groups were comparatively profiled utilizing isobaric tags fo ...[more]

PMID: 33627638

Similar Datasets

Pituitary proteomic response to CMS

Project description:We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the pituitary were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

| PXD025429 |

Hippocampal proteomics of CMS-treated rats

Project description:We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the hippocampus were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

| PXD015946 |

Hypothalamic proteomics of CMS-treated rats

Project description:We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the hypothalamic were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

2020-11-21 | PXD022715 |

Striatal proteomics of CMS-treated rats

Project description:We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the striatal were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

2021-05-19 | PXD026107 |

Olfactory bulb proteomics of CMS-treated rats

Project description:We used a chronic mild stress (CMS) protocol to separate susceptible-depression, susceptible-anxiety and insusceptible rat subgroups. Differential proteomes in the olfactory bulb were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

2021-04-12 | PXD023766 |

Intersectional analysis of chronic mild stress-induced lncRNA-mRNA interaction networks in rat hippocampus reveals potential anti-depression/anxiety drug targets

Project description:Despite studies providing insight into the neurobiology of chronic stress, depression and anxiety, long noncoding RNA (lncRNA)-mediated mechanisms underlying the common and distinct pathophysiology of these stress-induced disorders remain nonconclusive. In a previous study, we used the chronic mild stress paradigm to separate depression-susceptible, anxiety-susceptible and insusceptible rat subpopulations. In the current study, lncRNA and messenger RNA (mRNA) expression was comparatively profiled in the hippocampus of the three stress groups using microarray technology. Groupwise comparisons identified distinct sets of lncRNAs and mRNAs associated with the three different behavioral phenotypes of the stressed rats. To investigate the regulatory roles of the dysregulated lncRNAs upon mRNA expression, correlations between the differential lncRNAs and mRNAs were first analyzed by combined use of weighted gene coexpression network analysis and ceRNA theory-based methods. Subsequent functional analysis of strongly correlated mRNAs indicated that the dysregulated lncRNAs were involved in various biological pathways and processes to specifically induce rat susceptibility or resiliency to depression or anxiety. Further intersectional analysis of phenotype-associated and drug-associated lncRNA-mRNA networks and subnetworks assisted in identifying 16 hub lncRNAs as potential targets of anti-depression/anxiety drugs. Collectively, our study established the molecular basis for understanding the similarities and differences in pathophysiological mechanisms underlying stress-induced depression or anxiety and stress resiliency, revealing several important lncRNAs that represent potentially new therapeutic drug targets for depression and anxiety disorders.

2020-08-21 | GSE156580 | GEO

Gene expression analysis of prefrontal cortex in mice exposed to chronic mild stress

Project description:Major depressive disorder (MDD) is a common disorder and is responsible for considerable disability in global functioning, anorexia, and severer medical comorbidity. Recently, some reports showed the relationship between MDD and the metabolic disorders such as diabetes. We examined gene expression profiles in the mice prefrontal cortex using genome-wide microarray technology, and determined gene expression profiles with and without chronic mild stress(CMS) for 4 weeks which was often used to make models of depression. To analyze the candidate genes involved in not only depression but dysfunction of physiological homeostasis like diabetes, we campared the gene expression levels between with and without CMS, then we isolated 494 genes showing a more than 2-fold increase or a less than 1/2-fold decrease, in addition, we chose the isolated genes transcriptional products of both samples were confirmed clearly. The prefrontal cortex of C57Bl/6 N sea mice with and without CMS. We mixed tatal RNA from 7 mice prefrontal cortex per each.

2014-02-28 | E-GEOD-49867 | biostudies-arrayexpress

Proteomic response of rat hippocampus under chronic unpredicted mild stress reveals insights into susceptibility and insensitivity to depression

Project description:We used a chronic unpredicted mild stress (CUMS) protocol to separate depression-susceptible and stress-insensitive rat subgroups. Differential proteomes in the hippocampus were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

2022-02-17 | PXD025773 | Pride

Animal proteomics of depression

Project description:The amygdala plays a key role in the pathophysiology of depression, but the molecular mechanisms underlying amygdalar hyperactivity in depression remain unclear. In this study, we used a chronic mild stress (CMS) protocol to separate susceptible and insusceptible rat subgroups. Proteomes in the amygdalae were analyzed differentially across subgroups based on labeling with isobaric tags for relative and absolute quantitation (iTRAQ) combined with mass spectrometry. Of 2562 quantified proteins, 102 were differentially expressed. Several proteins that might be associated with the stress insusceptibility/susceptibility difference, including synapse-related proteins, were identified in the amygdala. Immunoblot analysis identified changes in VGluT1, NMDA GluN2A and GluN2B and AMPA GluA1 receptors, and PSD-95, suggesting that CMS perturbs glutamatergic transmission in the amygdala. Changes in these regulatory and structural proteins provide insight into the molecular mechanisms underlying the abnormal synaptic morphological and functional plasticity in the amygdalae of stress-susceptible rats. Interestingly, the expression level of CaMKIIβ, potentially involved in regulation of glutamatergic transmission, was significantly increased in the susceptible group. Subsequent in vitro experimentation showed that overexpression of CaMKIIβ increased the expression of PSD-95 and GluA1 in cultured hippocampal neurons. This result suggested that CaMKIIβ functions upstream from PSD-95 and GluA1 to affect LTP-based postsynaptic functional plasticity in the amygdalae of susceptible rats. Therefore, amygdalar CaMKIIβ is a potential antidepressant target. Collectively, our findings contribute to a better understanding of amygdalar synaptic plasticity in depression.

2019-01-15 | PXD044124 |

Gene expression analysis of prefrontal cortex in mice exposed to chronic mild stress

2014-02-28 | GSE49867 | GEO