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Chemoproteomic Mapping of the Glycolytic Targetome in Cancer Cells

ABSTRACT: we report a Target Responsive Accessibility Profiling (TRAP) approach for the global and quantitative analysis of glycolytic metabolites targetome in native cancer cell milieu.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hui Ye

PROVIDER: PXD022568 | iProX | Tue Nov 17 00:00:00 GMT 2020

REPOSITORIES: iProX

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Chemoproteomic mapping of the glycolytic targetome in cancer cells.

Tian Yang Y Wan Ning N Zhang Hanqing H Shao Chang C Ding Ming M Bao Qiuyu Q Hu Haiyang H Sun Huiyong H Liu Chenguang C Zhou Kun K Chen Shuai S Wang Guangji G Ye Hui H Hao Haiping H

Nature chemical biology 20230615 12

Hyperactivated glycolysis is a metabolic hallmark of most cancer cells. Although sporadic information has revealed that glycolytic metabolites possess nonmetabolic functions as signaling molecules, how these metabolites interact with and functionally regulate their binding targets remains largely elusive. Here, we introduce a target-responsive accessibility profiling (TRAP) approach that measures changes in ligand binding-induced accessibility for target identification by globally labeling react ...[more]

PMID: 37322158

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