Proteomics

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Differential proteomic analysis reveals the mechanism of Providencia.sp to selenium and manganese stress


ABSTRACT: In this study, iTRAQ technique is employed to reveal the proteomic response of Providencia. sp under selenium and manganese stress. After trypsin digestion, peptide was desalted by Strata X C18 SPE column (Phenomenex) and vacuum-dried. Peptide was reconstituted in 0.5 M TEAB and processed according to the manufacturer’s protocol for TMT kit/iTRAQ kit. Then, The tryptic peptides were fractionated into fractions by high pH reverse-phase HPLC using Agilent 300Extend C18 column. Finally, The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC.

ORGANISM(S): Providencia Rettgeri 947

SUBMITTER: LiFang wu  

PROVIDER: PXD022749 | iProX | Thu Nov 26 00:00:00 GMT 2020

REPOSITORIES: iProX

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