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O-GlcNAc sites identification and quantification proteomics analysis of O-GlcNAc chromatin in MCF-7 and MCF-7/ADR cells

ABSTRACT: We used a metabolic labeling (GalNAz) based O-GlcNAc sites identification and quantification proteomics method to compare the O-GlcNAc chromatin of MCF-7 and MCF-7/ADR cells. The O-GlcNAc chromatin-associated proteins can be metabolically labeled with azides, followed by reaction with a cleavable DADPS (dialkoxydiphenylsilane) alkyne-biotin linker and enrichment with streptavidin beads. After cleavage of DADPS by formic acid, O-GlcNAc modified peptides were quantitative analysis using tandem mass spectroscopy (MS).

ORGANISM(S): Homo Sapiens

SUBMITTER: Yubo Liu

PROVIDER: PXD022857 | iProX | Tue Dec 01 00:00:00 GMT 2020

REPOSITORIES: iProX

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