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Global effects of RAB3GAP1 dysexpression on the proteome of mouse cortical neurons

ABSTRACT: The transfection of the in vitro neurons was conducted using the lentivirus for under-expression (partial silencing) or over-expression of RAB3GAP1 to separate UE-RAB3GAP1 (RAB3GAP1 under-expression), UE-Ctrl (over-expression control), OE-RAB3GAP1 (RAB3GAP1 over-expression), and OE-Ctrl (under-expression control) groups. Differential proteomes were analyzed based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with mass spectrometry.

ORGANISM(S): Mus Musculus

SUBMITTER: Jian Zhou

PROVIDER: PXD023412 | iProX | Mon Jan 04 00:00:00 GMT 2021

REPOSITORIES: iProX

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Global effects of RAB3GAP1 dysexpression on the proteome of mouse cortical neurons.

Liu Yanchen Y Tian Fenfang F Li Shuiming S Chen Wei W Gong Weibo W Xie Hong H Liu Dan D Huang Rongzhong R Liao Wei W Yi Faping F Zhou Jian J

Amino acids 20210807 9

Mounting studies have demonstrated that RAB3GAP1 expression is modified in brain diseases with multiple neurobiological functions and processes and acts as a potentially significant target. However, the cellular and molecular events arising from RAB3GAP1 dysexpression are still incompletely understood. In this work, underexpression and overexpression of RAB3GAP1 were first induced into cultured mouse cortical neurons by transfection with lentivirus plasmids. Then we globally explored the effects ...[more]

PMID: 34363538

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