Proteomic analysis in the study of the mitochondrial supercomplex assembly
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Jianxin Lyu
PROVIDER: PXD023474 | iProX | Fri Jan 08 00:00:00 GMT 2021
REPOSITORIES: iProX
Publications
A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.
Fang Hezhi H Ye Xianglai X Xie Jie J Li Yuanyuan Y Li Haiyan H Bao Xinzhu X Yang Yue Y Lin Zifan Z Jia Manli M Han Qing Q Zhu Jingjing J Li Xueyun X Zhao Qiongya Q Yang Yanling Y Lyu Jianxin J
Cell reports 20210401 2
The assembly pathways of mitochondrial respirasome (supercomplex I+III<sub>2</sub>+IV) are not fully understood. Here, we show that an early sub-complex I assembly, rather than holo-complex I, is sufficient to initiate mitochondrial respirasome assembly. We find that a distal part of the membrane arm of complex I (P<sub>D</sub>-a module) is a scaffold for the incorporation of complexes III and IV to form a respirasome subcomplex. Depletion of P<sub>D</sub>-a, rather than other complex I modules, ...[more]
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