Dataset Information

Proteomic data of ovary of OHSS model rat

ABSTRACT: The mass spectrometer was used to identify the labeled samples from electroacupuncture treated and Ovarian Hyper-stimulation Syndrome (OHSS) group. The ovarian proteins from four biological replicates in each group were extracted using the phenolic extraction method, iTRAQ labeling and quantitative LC-MS proteomics were performed according to the manufacturer's instructions. The quantitative protein ratios were weighted and normalized by the median ratio in Mascot. When the differences in protein expression between the two groups were >1.2-fold or <-1.2-fold (with P < 0.05), the protein was considered to be differentially expressed.

ORGANISM(S): Rattus Rattus

SUBMITTER: Bing Yao

PROVIDER: PXD023493 | iProX | Sun Jan 10 00:00:00 GMT 2021

REPOSITORIES: iProX

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Publications

Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200.

Chen Li L Huang Xuan X Wang Li L Wang Cencen C Tang Xu X Gu Minghui M Jing Jun J Ma Rujun R Ge Xie X Yao Bing B

Frontiers in cell and developmental biology 20210222

Ovarian hyperstimulation syndrome (OHSS) is a common complication caused by ovulatory stimulation therapy, which manifests as an increase in ovarian volume, an increase in the number of oocytes retrieved, and increased vascular permeability throughout the body and especially in ovarian tissue. In our previous study, we found that electroacupuncture (EA) could prevent the progression of OHSS, by mainly affecting ovary. However, the specific molecules and the mechanism of this process were still u ...[more]

PMID: 33693006

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Project description:To identify and quantify protein profiles from peripheral blood mononuclear cells (PBMC) of osteopetrosis patients with isobaric Tagging for Relative and Absolute protein Quantification (iTRAQ) based proteomic technology and to find differentially expressed proteins in osteopetrosis. Total protein was extracted from PBMC samples of 6 osteopetrosis patients and 9 health donors. For the osteopetrosis group and the control group, aliquots of protein from each subject were pooled respectively. One hundred micrograms of protein from each corresponding group pool protein was digested with trypsin, and labeled according to the iTRAQ protocol. The labeled digests were then combined into one sample mixture and subjected to LC-MS/MS analysis. Identification and relative quantification of proteins based on the ratio of peak areas from MS/MS spectra, and the m/z of 114 (osteopetrosis) and 115 (control) were involved in this research. The MS data were processed using Proteome Discoverer Software (Version 1.2.0.208) to generate the peak list. Mascot 2.3.02 was used for the protein identification, and the parameters as follows: Type of search: MS/MS Ion search; Enzyme: Trypsin; Fragment mass tolerance: ±0.1 Da; Mass values: Monoisotopic; Variable modifications: Gln->pyro-Glu (N-term Q), Oxidation (M), iTRAQ8plex(Y); Peptide mass tolerance: 0.05 Da; Instrument type: Defult; Max missed cleavages: 1; Fixed modifications: arbamidomethyl (C), iTRAQ8plex (N-term), iTRAQ8plex (K); Protein Mass: Unrestricted; Other parameters: default; Database, IPI_human v3.87 (91464 sequences). The search results were further filtered with the parameters as follows: for protein identification, significance threshold: P<0.05 (with 95% confidence), ion score or expected cutoff < 0.05 (with 95% confidence); for protein quantitation, protein ratio type: weighted; minimum precursor charge: 1; minimum peptides: 2 (unique peptides); median intensities: normalization (removed outliers automatically). Proteins with 1.5 fold or more change between osteopetrosis group and the control group were determined as differentially expressed proteins.

Dataset Information

Proteomic data of ovary of OHSS model rat

Publications

Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200.

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