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A novel SDS-based sample preparation for proteomics

ABSTRACT: Sodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Most of current SDS removal strategies are time-consuming, laborious and low reproducibility. Here, we describe a method, SDS-cyclodextrin assisted sample preparation (SCASP), by which cyclodextrin can bind to SDS and form cyclodextrins-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SCASP is a simple, fast and robust SDS-based sample preparation method for proteomics application.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Chuan-Qi Zhong

PROVIDER: PXD023557 | iProX | Tue Mar 02 00:00:00 GMT 2021

REPOSITORIES: iProX

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SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research.

Gan Guohong G Xu Xiao X Chen Xi X Zhang Xiu-Fang XF Wang Jinling J Zhong Chuan-Qi CQ

Molecular & cellular proteomics : MCP 20210205

SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC-MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS-cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SD ...[more]

PMID: 33549647

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