Dataset Information

A BioID-mediated proximity-labeling of human DTX2

ABSTRACT: To identify human DTX2 proximal proteome, the BioID-DTX2 fusion protein was expressed in DTX2 KO HeLa cells. The biotinylated proteins were pulled down and subjected to mass spectrometry analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Songyang Zhou

PROVIDER: PXD023795 | iProX | Tue Oct 11 00:00:00 BST 2022

REPOSITORIES: iProX

ACCESS DATA

Publications

An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomerase.

Zhou Zhifen Z Li Yujing Y Xu Huimin H Xie Xiaowei X He Zibin Z Lin Song S Li Ruofei R Jin Shouheng S Cui Jun J Hu Hai H Liu Feng F Wu Su S Ma Wenbin W Songyang Zhou Z

iScience 20220125 2

Most tumor cells reactivate telomerase to ensure unlimited proliferation, whereas the expression of human telomerase reverse transcriptase (hTERT) is tightly regulated and rate-limiting for telomerase activity maintenance. Several general transcription factors (TFs) have been found in regulating hTERT transcription; however, a systematic study is lacking. Here we performed an inducible CRISPR/Cas9 KO screen using an hTERT core promoter-driven reporter. We identified numerous ...[more]

PMID: 35198878

Similar Datasets

Project description:Classical methods of investigating protein-protein interactions (PPIs) are generally performed in non-living systems, yet in recent years new technologies utilizing proximity labeling (PL) have given researchers the tools to explore proximal PPIs in living systems. PL has distinct advantages over traditional protein interactome studies, such as the ability to identify weak and transient interactions in vitro and in vivo. Most PL studies are performed on targets within or on the cell membrane. We have adapted the original PL method to investigate PPIs within the extracellular compartment, using both BioID2 and TurboID, that we term extracellular PL (ePL). To demonstrate the utility of this modified technique, we investigate the interactome of the widely expressed matrisome protein Tissue inhibitors of metalloproteinases 2 (TIMP2). Tissue inhibitors of metalloproteinases (TIMPs) are a family of multi-functional proteins that were initially defined by their ability to inhibit the enzymatic activity of metalloproteinases (MPs), the major mediators of extracellular matrix (ECM) breakdown and turnover. TIMP2 exhibits a broad expression profile and is often abundant in both normal and diseased tissues. Understanding the functional transformation of matrisome regulators, like TIMP2, during the evolution of tissue microenvironments associated with disease progression is essential for the development of ECM targeted therapeutics. Using carboxyl- and amino-terminal fusion proteins of TIMP2 with BioID2 and TurboID, we describe the TIMP2 proximal interactome. We also illustrate how the TIMP2 interactome changes in the presence of different stimuli, in different cell types, in unique culture conditions (2D vs 3D), and with different reaction kinetics (BioID2 vs. TurboID); demonstrating the power of this technique versus classical PPI methods. We propose that the screening of matrisome targets in disease models using ePL will reveal new therapeutic targets for further comprehensive studies.

Dataset Information

A BioID-mediated proximity-labeling of human DTX2

Publications

An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomerase.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure