Dataset Information

Effect of water activity in Aspergillus flavus spore germination

ABSTRACT: In this study, we investigated the effect of water activity on transcriptome and proteome of Aspergillus flavus spore germination.we investigated the most important protein in PPI network in proteome, the S-adenosylmethionine-dependent methyltransferase superfamily domain-containing protein. Studies on S-adenosylmethionine (SAM)-dependent methyltransferases showed that it is crucial in cell signaling, metabolism and epigenetic programming

ORGANISM(S): Aspergillus Flavus Nrrl3357

SUBMITTER: Desheng Qi

PROVIDER: PXD023808 | iProX | Tue Jan 26 00:00:00 GMT 2021

REPOSITORIES: iProX

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Publications

Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i>.

Jia Sifan S Li Chong C Wu Kuntan K Qi Desheng D Wang Shuai S

Microorganisms 20220829 9

In this study, we explored the mechanism underlying <i>Aspergillus flavus</i> conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decreased cell sizes. Transcriptomics found numerous differentially expressed genes (DEGs) during the first 12 h of germination, with 654 DEGs observed among 4 h, 8 h and 12 h. In particular, more DEGs were detected a ...[more]

PMID: 36144346

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Project description:Aspergillus flavus is a common saprophyte and opportunistic pathogen producing aflatoxin (AF) and many other secondary metabolites. 5-Azacytidine (5-AC), a derivative of nucleoside cytidine, is widely used for studies in epigenetics and cancer biology as an inactivator of DNA methyltransferase and is also used for studying secondary metabolism in fungi. Our previous studies showed that 5-AC affects development and inhibits AF production in A. flavus, and that A. flavus lacks DNA methylation. How this common DNA methyltransferase inhibitor affects development and AF production is not clear. In this study, we applied an RNA-Seq approach to elucidate the mechanism of 5-ACM-bM-^@M-^Ys effect on A. flavus. In our current study, we identified 240 significantly differently expressed (Q-value<0.05) genes after 5-AC treatment, including two backbone genes in secondary metabolite clusters #27 and #35, which are involved in development or survival of sclerotia. With 5-AC treatment, about three quarters of the genes in the AF biosynthetic gene cluster in A. flavus were down-regulated to a certain degree. Strikingly, at least two genes aflI and aflLa, were completely inhibited. Interestingly, several genes involved in fungal development were down-regulated, especially veA, which is a gene that encodes protein bridges VelB and LaeA. This result supports the hypothesis that 5-AC affects development and AF production through weakening or even interrupting the connection between VelB and LaeA and then causing dysregulation of the expression pattern of genes involved in development and secondary metabolism. Our results improved the A. flavus genome annotation, provided a comprehensive view of the transcriptome of A. flavus responding to 5-AC and confirmed that fungal development and secondary metabolism are co-regulated. In additon, the RNA-Seq data of another sample treated with gallic acid was used to improve A. flavus genome annotation. mRNA of Aspergillus flavus cultured in three different culture media PDB, PDB+5-AC(5-Azacytidine),and PDB+GA(gallic acid) was subjected to sequence independently.

Dataset Information

Effect of water activity in Aspergillus flavus spore germination

Publications

Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i>.

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