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The comprehensive stage-specific proteome across human pre-implantation embryo development at single-cell level

ABSTRACT: Although the landscape of epigenomic and transcriptomic regulation during human pre-implantation development has been depicted by applying single-cell sequencing, the investigation of embryonic proteome is almost unrevealed due to the insufficient input quantity from precious human embryonic samples for traditional mass spectrometry (MS). With applying the state-of-the-art ultrahigh sensitivity mass spectrometry technology and nanoliter-scale oil-air-droplet (OAD) chip, we were able to identify more than three thousand proteins in a single oocytes during human pre-implantation development. Hundreds of stage-specific proteins with significant expressional changes were classified. Many that involve important functions, such as paternal genome reprogramming or DNA methylation, were firstly identified in human embryo studies. We discovered a two peaks of “zygotic proteome activation” (ZPA) at 2-cell and morula stages, in which proteins are mainly associated with epigenetic reprogramming. Our study, for the first time, delineates a comprehensive stage-specific proteome landscape at a single-cell level. It is as well a large step of enriching the panorama of functional genomics across human pre-implantation embryo development.

ORGANISM(S): Homo Sapiens

SUBMITTER: Catherine CL Wong

PROVIDER: PXD024267 | iProX | Tue Apr 20 00:00:00 BST 2021

REPOSITORIES: iProX

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Functional profiling of stage-specific proteome and translational transition across human pre-implantation embryo development at a single-cell resolution.

Dang Yujiao Y Zhu Liu L Yuan Peng P Liu Qiang Q Guo Qianying Q Chen Xi X Gao Shuaixin S Liu Xiao X Ji Shushen S Yuan Yifeng Y Lian Ying Y Li Rong R Yan Liying L Wong Catherine C L CCL Qiao Jie J

Cell discovery 20230124 1

PMID: 36693841

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