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Online Microfluidics Mass Spectrometry Reveals Molecular Responses of Bacterial Filamentation and Inhibition Methods of Drug Resistant Bacteria

ABSTRACT: Filamentation is one of the self-saving responses and a survival strategy of bacteria in the circumstance of antibiotics. Understanding the molecular mechanism of filamentation can lead to the development of new inhibition methods for antibiotic-resistant bacteria. In this work, we develop an online microfluidics mass spectrometry for real‐time analysis of metabolic changes of bacteria during filamentation under the stimulus of antibiotics. Together with untargeted proteomic analyses and fluorescence imaging of target enzymes, significant pathways, e.g. nucleotide metabolism and coenzyme A biosynthesis, correlated to the filamentation of extended spectrum beta‐lactamase producing E. coli (ESBL-E. coli) are identified.

ORGANISM(S): Escherichia Coli

SUBMITTER: Liang Qiao

PROVIDER: PXD024394 | iProX | Fri Feb 26 00:00:00 GMT 2021

REPOSITORIES: iProX

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Molecular responses during bacterial filamentation reveal inhibition methods of drug-resistant bacteria.

Zhang Dongxue D Yin Fan F Qin Qin Q Qiao Liang L

Proceedings of the National Academy of Sciences of the United States of America 20230626 27

Bacterial antimicrobial resistance (AMR) is among the most significant challenges to current human society. Exposing bacteria to antibiotics can activate their self-saving responses, e.g., filamentation, leading to the development of bacterial AMR. Understanding the molecular changes during the self-saving responses can reveal new inhibition methods of drug-resistant bacteria. Herein, we used an online microfluidics mass spectrometry system for real-time characterization of metabolic changes of ...[more]

PMID: 37364094

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