Dataset Information

Liver proteomics investigations into hypothyroidism treated with Atractylodis Macrocephalae Rhizome

ABSTRACT: Hypothyroidism model rats were established with PTU (10 mg•kg-1•d-1) and treated with Atractylodis Macrocephalae Rhizome and its fractions (volatile oil fraction, lactones fraction, atractyloside fraction, oligosaccharide fraction, and crude polysaccharide fraction) for 20 d. The experiment (having been) finished, liver samples were gained. Protein concentrations measured by the BCA kit and the tryptic peptides obtained from the different groups were labeled with iTRAQ reagents. The peptides were eluted into 10 fractions by High-pH Reverse-phase Liquid Chromatography Fractionation and analyzed by LC-ESI-MS/MS Analysis based on Triple TOF 5600. Results indicated that these DEPs contained Pygl, Pklr, Pc, Ehhadh, Acox1, Fasn, Acly, Acsl1, Acsl5, Pgm1, Suclg1, Gpt, Idh1, Fh, and Adh1, which could represent target proteins of Atractylodis Macrocephalae Rhizome and its fractions for hypothyroidism. Atractylodis Macrocephalae Rhizome and its fractions played roles in regulating the substance and energy metabolism for the treatment of hypothyroidism.

ORGANISM(S): Rattus Rattus

SUBMITTER: Deqiang Dou

PROVIDER: PXD024535 | iProX | Thu Mar 04 00:00:00 GMT 2021

REPOSITORIES: iProX

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Publications

Application of Proteomics and Metabonomics to Reveal the Molecular Basis of Atractylodis Macrocephalae Rhizome for Ameliorating Hypothyroidism Instead of Hyperthyroidism.

Chen Jing J Dou Peiyuan P Xiao Hang H Dou Deqiang D Han Xueying X Kuang Haixue H

Frontiers in pharmacology 20210420

As the treatments of diseases with Chinese herbs are holistic and characterized by multiple components, pathways, and targets, elucidating the efficacy of Chinese herbs in treating diseases, and their molecular basis, requires a comprehensive, network-based approach. In this study, we used a network pharmacology strategy, as well as <i>in vivo</i> proteomics and metabonomics, to reveal the molecular basis by which <i>Atractylodis macrocephalae</i> rhizome (AMR) ameliorates hypothyroidism. Eighte ...[more]

PMID: 33959028

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Dataset Information

Liver proteomics investigations into hypothyroidism treated with Atractylodis Macrocephalae Rhizome

Publications

Application of Proteomics and Metabonomics to Reveal the Molecular Basis of Atractylodis Macrocephalae Rhizome for Ameliorating Hypothyroidism Instead of Hyperthyroidism.

Similar Datasets

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