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Free-Flow Isoelectric Focusing for Comprehensive Separation and Analysis of Human Salivary Microbiome for Lung Cancer

ABSTRACT: Human microbiome contains billions of microorganisms that play important roles in the biological system and different diseases. Due to its complexity, conventional culture-independent technology may underestimate the value of low-abundance bacteria, which calls for a highly efficient method for its enrichment and comprehensive analysis. In this study, we developed a recycling free-flow isoelectric focusing (RFFIEF) method-based electrophoresis method to separate salivary microbiome.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hua Xiao

PROVIDER: PXD024619 | iProX | Wed Mar 10 00:00:00 GMT 2021

REPOSITORIES: iProX

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Free-Flow Isoelectric Focusing for Comprehensive Separation and Analysis of Human Salivary Microbiome for Lung Cancer.

Jiang Xiaoteng X Liu Sha S Zhang Yan Y Ji Yin Y Sohail Amir A Cao Chengxi C Wang Peng P Xiao Hua H

Analytical chemistry 20200818 17

Human microbiome contains billions of microorganisms that play important roles in the biological system and different diseases. Due to its complexity, conventional culture-independent technology may underestimate the value of low-abundance bacteria, which calls for a highly efficient method for its enrichment and comprehensive analysis. In this study, we developed a recycling free-flow isoelectric focusing (RFFIEF) method-based electrophoresis method to separate salivary microbiome. First, we us ...[more]

PMID: 32786473

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