Comparative secretome analysis of Magnaporthe oryzae identified N-glycosylation proteins involved in fungal pathogenicity and cell wall integrity

ABSTRACT: Plant fungal pathogens secrete numerous proteins into the apoplast at the plant-fungus contact sites to facilitate colonization. Only a limited number of secreted proteins have been functionally characterized in Magnaporthe oryzae, the causal agent of blast disease in rice (Oryza sativa). Intriguingly, the ∆alg3 mutant of M. oryzae, which -1, 3-mannosyltransferase ALG3, shows strong defects in fungal cell wall integrity and virulence, indicating a potential effect on the secretion of multiple proteins. Therefore, in this study, we used a proteomics approach, comparing wild type and ∆alg3 mutants, to identify 51 proteins that require ALG3 for proper secretion. Elucidation of the comparative secretome of M. oryzae improves our understanding of the proteins that require ALG3 for secretion, and of their function in fungal virulence and cell wall integrity.

ORGANISM(S): Magnaporthe Oryzae P131

SUBMITTER: Xunli LU  

PROVIDER: PXD024706 | iProX | Fri Mar 12 00:00:00 GMT 2021

REPOSITORIES: iProX