Dataset Information

Proteome landscape of mouse oocyte mitochondria

ABSTRACT: Mitochondria are essential organelles that provide energy for mammalian cells, with multiple functions such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional layer of importance since they are required for germ cell maturation, dysfunctions of which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed for basic and clinical research, but has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes. Here, in this work, using proximity labeling proteomics, we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV staged oocytes of mouse and and explore oocyte mitochondrial proteomics.

ORGANISM(S): Mus Musculus

SUBMITTER: Mo Li

PROVIDER: PXD024777 | iProX | Tue Mar 16 00:00:00 GMT 2021

REPOSITORIES: iProX

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Publications

Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile.

Zhang Na N Sun An-di AD Sun Si-Man SM Yang Rui R Shi Yan-Yan YY Wang Qi-Long QL Li Xin-Yu XY Ma Ji-Hong JH Yue Wei W Xie Bing-Teng BT Qiao Jie J Li Mo M

Acta pharmacologica Sinica 20210520 12

Mitochondria are essential organelles that provide energy for mammalian cells and participate in multiple functions, such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional level of importance since they are required for germ cell maturation, dysfunction in which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed t ...[more]

PMID: 34017067

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Project description:Background: Early embryonic development is governed by maternal transcripts stored within the oocyte during oogenesis. Transcriptional activity of the oocyte ultimately dictates its developmental potential and may be influenced by maternal age, resulting in reduced competence of oocytes derived from women of advanced age, compared with the young. In the current study, RNA-Seq was used to perform transcriptome profiling of human GV and MII oocytes derived from young and advanced maternal age women. Participants/Materials and Methods: Cumulus dissection from donated oocytes was performed. GV and MII oocytes underwent deep RNA sequencing using the SMART-Seq v4 Ultra Low Input RNA protocol (Takara-Clontech, USA) and Nextera XT DNA library preparation kit (Illumina, USA). Data processing, quality assessment and bioinformatics analysis were performed using source-software, including FastQC, HISAT2, StringTie, edgeR and DAVID. Results: Following deep single-cell RNA-Seq on GV and MII oocytes, hundreds of transcripts were significantly differentially expressed between young maternal age (YMA) and advanced maternal age (AMA) groups, with the most significant biological processes relating to mitochondrial reserves. The GV to MII transition shares common biological processes between young and AMA groups, however, some genes involved in mitochondria function were altered during ageing. A decrease in mitochondrial-related transcripts was also observed during the GV to MII transition. However, there was a much greater reduction of mitochondrial-related transcripts in MII oocytes of AMA. This observation was confirmed when YMA MII oocytes were compared with the AMA MII group with mitochondrial-related transcripts being significantly higher expressed in the YMA group, including biological processes, such as mitochondrial electron transport and ATP biosynthetic process. These results indicate a higher energy potential in YMA MII oocytes that is decreased with age. Other significantly higher biological processes in the YMA MII group include transcripts involved in the regulation of ubiquitin-dependent degradation. Lack of these transcripts could lead to a non-appropriate removal of oogenesis remnants following fertilisation in the AMA MII group. Discussion: Understanding reproductive ageing effects at the RNA level in human oocytes may reveal differences in the mechanisms regulating the GV to MII transition that impact on oocyte quality in YMA and AMA patients. Further investigations of the up-/down-regulated transcripts during ageing could guide and improved IVF outcomes for AMA patients.

Dataset Information

Proteome landscape of mouse oocyte mitochondria

Publications

Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile.

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