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Phosphoproteomics analysis in mouse primary hepatocytes with recombinant GP73 or glucagon

ABSTRACT: Isolated mouse primary hepatocytes were cultured at glucose- and phenol-free DMEM medium, supplemented with 10mM pyruvate sodium and 10mM sodium lactate, and the cells were incubated for 1hr with vehice, recombinant mGP73 (64nM), or glucagon (3μM) ,cells were washed twice with cold PBS and scraped with cold RIPA lysis buffer supplemented with proteases and phosphatases inhibitors. Phosphoproteomics assay was performed by Shanghai luming biological technology co., LTD (Shanghai, China).

ORGANISM(S): Mus Musculus

SUBMITTER: Hui Zhong

PROVIDER: PXD025381 | iProX | Thu Nov 18 00:00:00 GMT 2021

REPOSITORIES: iProX

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