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Label-free quantitative proteomics for screening dynamic autophagy-related proteins during nutrient deprivation in MEFs

ABSTRACT: A Label-free quantitative proteomic profiling was performed on MEFs with or without nutrient starvation.

ORGANISM(S): Mus Musculus

SUBMITTER: Dong Xiao

PROVIDER: PXD025450 | iProX | Sat Oct 15 00:00:00 BST 2022

REPOSITORIES: iProX

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Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA.

Hao WeiChao W Dian MeiJuan M Zhou Ying Y Zhong QiuLing Q Pang WenQian W Li ZiJian Z Zhao YaYan Y Ma JiaCheng J Lin XiaoLin X Luo RenRu R Li YongLong Y Jia JunShuang J Shen HongFen H Huang ShiHao S Dai GuanQi G Wang JiaHong J Sun Yan Y Xiao Dong D

Nature communications 20221004 1

Autophagy is crucial for maintaining cellular energy homeostasis and for cells to adapt to nutrient deficiency, and nutrient sensors regulating autophagy have been reported previously. However, the role of eiptranscriptomic modifications such as m6A in the regulation of starvation-induced autophagy is unclear. Here, we show that the m6A reader YTHDF3 is essential for autophagy induction. m6A modification is up-regulated to promote autophagosome formation and lyso ...[more]

PMID: 36195598

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