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Identification of Silkworm Organelle Proteome

ABSTRACT: Isolate silkworm proteins in organelles of nucleus, cytosol, mitochondria and endoplasmic reticulum , and determine the proteins using mass spectrum

ORGANISM(S): Bombyx Mori

SUBMITTER: Zeyang Zhou

PROVIDER: PXD025816 | iProX | Thu May 06 00:00:00 BST 2021

REPOSITORIES: iProX

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Proteomic Identification of Bombyx mori Organelles Using the Engineered Ascorbate Peroxidase APEX and Development of Silkworm Organelle Proteome Database (SilkOrganPDB).

Li Tian T Xu Chen C Xu Jinzhi J Luo Jian J Yu Bin B Meng Xianzhi X Li Chunfeng C Pan Guoqing G Zhou Zeyang Z

International journal of molecular sciences 20210510 9

Silkworm Bombyx mori is an economically important insect and a lepidopteran model. Organelle proteome is vital to understanding gene functions; however, it remains to be identified in silkworm. Here, using the engineered ascorbate peroxidase APEX, we constructed transgenic B. mori embryo cells (BmE) expressing APEX-NLS, COX4-APEX, APEX-Rev, and APEX-KDEL in nucleus, mitochondrial matrix (MM), cytosol, and endoplasmic reticulum (ER), and isolated the biotin-labeled proteins using st ...[more]

PMID: 34068790

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