Global Profiling of RNA-Binding Protein Target Sites by LACE-seq
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus Musculus
SUBMITTER: 
Yuanchao Xue
PROVIDER: PXD025846 | iProX | Fri May 07 00:00:00 BST 2021
REPOSITORIES: iProX
Publications
Global profiling of RNA-binding protein target sites by LACE-seq.
Su Ruibao R Fan Li-Hua LH Cao Changchang C Wang Lei L Du Zongchang Z Cai Zhaokui Z Ouyang Ying-Chun YC Wang Yue Y Zhou Qian Q Wu Ligang L Zhang Nan N Zhu Xiaoxiao X Lei Wen-Long WL Zhao Hailian H Tian Yong Y He Shunmin S Wong Catherine C L CCL Sun Qing-Yuan QY Xue Yuanchao Y
Nature cell biology 20210609 6
RNA-binding proteins (RBPs) have essential functions during germline and early embryo development. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance cells. Here, by coupling RBP-mediated reverse transcription termination with linear amplification of complementary DNA ends and sequencing, we present the LACE-seq method for identifying RBP-regulated RNA networks at or near the single-oocyte level. We determined the binding sites and regulatory mech ...[more]
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