Dataset Information

Urine Metabolic Alternation of Acute Kidney Injury after Cardiac Surgery using UltraHigh-Performance Liquid Chromatography with High-Resolution Mass Spectrometry

ABSTRACT: Non-targeted urine metabolomics of cardiac surgery-induced AKI patients at different time point was carried out based on ultrahigh-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometer (UPLC-QE MS) technology.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chunbo Chen

PROVIDER: PXD025917 | iProX | Mon May 10 00:00:00 BST 2021

REPOSITORIES: iProX

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Use of ultra high performance liquid chromatography with high resolution mass spectrometry to analyze urinary metabolome alterations following acute kidney injury in post-cardiac surgery patients.

Bai Yunpeng Y Zhang Huidan H Wu Zheng Z Huang Sumei S Luo Zhidan Z Wu Kunyong K Hu Linhui L Chen Chunbo C

Journal of mass spectrometry and advances in the clinical lab 20220222

<h4>Background</h4>Cardiac surgery-associated acute kidney injury (AKI) can increase the mortality and morbidity, and the incidence of chronic kidney disease, in critically ill survivors. The purpose of this research was to investigate possible links between urinary metabolic changes and cardiac surgery-associated AKI.<h4>Methods</h4>Using ultra-high-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry, non-targeted metabolomics was performed on urinary samples co ...[more]

PMID: 35252948

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Project description:Objectives: Secretion of extracellular vesicles (EV) and associated micro-RNAs (miR) is altered during cellular stress and may serve as biomarkers of organ injury. We hypothesized that measuring changes in urinary levels of EV and miR will predict the onset of acute kidney injury in cardiac surgery patients. Design: Predictive accuracy biomarker study performed in the cohort of the REVAKI-2 trial Setting: Single center ICU between September 2015 and September 2018 Interventions: Intravenous sildenafil citrate 12.5 mg kg-1 over 150 min or dextrose 5% at the commencement of surgery. Measurements and main results: Urine samples were collected before and 24 hours after the procedure from 93 cardiac surgery patients. Urine EV concentrations and size distribution were assessed using NanoSight. EV derivation and levels were measured using flow cytometry. Samples from 10 selected patients were sequenced to detect differentially expressed miR. Verification was performed with advanced TaqMan assays in samples from all patients. Urine EV concentrations significantly increased in patients with AKI after surgery, with the percentage of EV positive for aquaporin-2 and β1-integrin also increasing. Pre surgery podocalyxin-positive EV were significantly lower, and β1-integrin EV werehigher in patients with AKI. The levels of the former correlated with the severity of theinjury. miR-125a-5p was expressed at higher levels in urine from patients with AKI stage 2/3. Levels of miR-10a-5p decreased after surgery in AKI patients; its levels correlated with the severity of the injury. Preoperative levels of podocalyxin EVs and miR-125a-5p had moderate AKI predictive value and, in a logistic model together with ICU lactate levels, offered good (AUC = 80.9%) AKI prediction. Conclusions: Lower levels of podocalyxin-positive EV at baseline predict the severity of post-surgery AKI. Urine EV concentrations and miR expression offer excellent predictive accuracy when combined with commonly measured biomarkers.

Dataset Information

Urine Metabolic Alternation of Acute Kidney Injury after Cardiac Surgery using UltraHigh-Performance Liquid Chromatography with High-Resolution Mass Spectrometry

Publications

Use of ultra high performance liquid chromatography with high resolution mass spectrometry to analyze urinary metabolome alterations following acute kidney injury in post-cardiac surgery patients.

Similar Datasets

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