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Dichotomic size-exclusion chromatography（SEC）

ABSTRACT: directDIA proteomics raw data of plasma extracellular vesicles isolated by SEC and UC

ORGANISM(S): Homo Sapiens

SUBMITTER: Tong Wang

PROVIDER: PXD026034 | iProX | Mon May 17 00:00:00 BST 2021

REPOSITORIES: iProX

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Establishment of a simplified dichotomic size-exclusion chromatography for isolating extracellular vesicles toward clinical applications.

Guo Jiahui J Wu Caihong C Lin Xinyi X Zhou Jian J Zhang Jiayi J Zheng Wenting W Wang Tong T Cui Yizhi Y

Journal of extracellular vesicles 20210901 11

Size-exclusion chromatography (SEC) is a widely adopted method for the isolation of extracellular vesicles (EVs) from complex samples. SEC can efficiently remove high-abundant proteins, while often requires multiple fractionation operation using diversified column settings. In this study, we aim to establish a simplified SEC method to acquire high quality EVs. In comparison of all three cross-linked Sepharose resins with the sample types of FBS and human serum (HS), CL-6B and CL-4B showed superi ...[more]

PMID: 34514732

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