Proteomics

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M6A binding proteins identified by SPIDER assay


ABSTRACT: To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs(with and without m6A modification)with the total lysate of YTDHFs (YTHDF1,YTHDF2 and YTHDF3)-overexpressed HEK293T cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Shengce Tao  

PROVIDER: PXD026519 | iProX | Wed Nov 02 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications


Protein-biomolecule interactions play pivotal roles in almost all biological processes. For a biomolecule of interest, the identification of the interacting protein(s) is essential. For this need, although many assays are available, highly robust and reliable methods are always desired. By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin (SA)-biotin system, we developed the Specific Pupylation as IDEntity Repor  ...[more]

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