M6A binding proteins identified by SPIDER assay

ABSTRACT: To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and without m6A modification）with the total lysate of YTDHFs (YTHDF1,YTHDF2 and YTHDF3)-overexpressed HEK293T cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Shengce Tao  

PROVIDER: PXD026519 | iProX | Wed Nov 02 00:00:00 GMT 2022

REPOSITORIES: iProX