Proteomics

Dataset Information

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MRNA-protein interactome of THP-1 cells


ABSTRACT: To investigate the effectiveness of SPIDER identifying the mRNA interactome, we carried out the SPIDER assay by incubating biotin-oligo(dT) or biotin control directly with cell lysates of THP-1 cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Shengce Tao  

PROVIDER: PXD026521 | iProX | Fri Jun 04 00:00:00 BST 2021

REPOSITORIES: iProX

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Publications


Protein-biomolecule interactions play pivotal roles in almost all biological processes. For a biomolecule of interest, the identification of the interacting protein(s) is essential. For this need, although many assays are available, highly robust and reliable methods are always desired. By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin (SA)-biotin system, we developed the Specific Pupylation as IDEntity Repor  ...[more]

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