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Identification of ECT2-containing protein complex

ABSTRACT: Cellular extracts from HeLa cells expressing FLAG-ECT2 were immunopurified with anti-FLAG affinity beads and eluted with FLAG peptide. The eluates were resolved on SDS/PAGE and silver stained, followed by mass spectrometric analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenchen Gong

PROVIDER: PXD026977 | iProX | Tue Jun 29 00:00:00 BST 2021

REPOSITORIES: iProX

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Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

Cao Cheng C Han Peiyi P Liu Ling L Tang Yiman Y Tian Shanshan S Zhang Kai K Shi Lei L Liu Zhiqiang Z Zhuo Dexiang D Ge Wenshu W Gong Wenchen W

The Journal of biological chemistry 20210731 3

Proteins containing breast cancer type 1 (BRCA1) C-terminal domains play crucial roles in response to and repair of DNA damage. Epithelial cell transforming factor (epithelial cell transforming sequence 2 [ECT2]) is a member of the BRCA1 C-terminal protein family, but it is not known if ECT2 directly contributes to DNA repair. In this study, we report that ECT2 is recruited to DNA lesions in a poly (ADP-ribose) polymerase 1-dependent manner. Using co-immunoprecipitation analysis, we showed that ...[more]

PMID: 34343566

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