Spatially region resolved proteomic map reveals extracellular matrix regulates epidermal growth and function as epidermal stem cell niche

ABSTRACT: Spatial proteins expression is critical to identify exactly protein localization and function in tissues. By combining decellularization, laser-capture microdissection, and mass spectrometry, six different partitioned proteins among skin layers of stratum corneum (SC), granular-spinous (GS), basal layer (BL), BM, superficial dermis (SD), and deep dermis (DD) were isolated, enriched and for the first time, a stratified and developmental lineage proteome map of human skin has been revealed spatially. Especially, we found TGFβ induced (TGFBI), an ECM glycoprotein, in the BM that enhances the proliferation of EpSCs and promotes the process of wound healing. Most interestingly, TGFBI shows the potential for treating secondary syphilis patients, which may benefit clinical practice.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yunping Zhu  

PROVIDER: PXD027093 | iProX | Mon Jul 05 00:00:00 BST 2021

REPOSITORIES: iProX