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Spatially region resolved proteomic map reveals extracellular matrix regulates epidermal growth and function as epidermal stem cell niche

ABSTRACT: Spatial proteins expression is critical to identify exactly protein localization and function in tissues. By combining decellularization, laser-capture microdissection, and mass spectrometry, six different partitioned proteins among skin layers of stratum corneum (SC), granular-spinous (GS), basal layer (BL), BM, superficial dermis (SD), and deep dermis (DD) were isolated, enriched and for the first time, a stratified and developmental lineage proteome map of human skin has been revealed spatially. Especially, we found TGFβ induced (TGFBI), an ECM glycoprotein, in the BM that enhances the proliferation of EpSCs and promotes the process of wound healing. Most interestingly, TGFBI shows the potential for treating secondary syphilis patients, which may benefit clinical practice.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yunping Zhu

PROVIDER: PXD027093 | iProX | Mon Jul 05 00:00:00 BST 2021

REPOSITORIES: iProX

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Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth.

Li Jun J Ma Jie J Zhang Qiyu Q Gong Huizi H Gao Dunqin D Wang Yujie Y Li Biyou B Li Xiao X Zheng Heyi H Wu Zhihong Z Zhu Yunping Y Leng Ling L

Nature communications 20220711 1

Human skin comprises stratified squamous epithelium and dermis with various stromal cells and the extracellular matrix (ECM). The basement membrane (BM), a thin layer at the top of the dermis, serves as a unique niche for determining the fate of epidermal stem cells (EpSCs) by transmitting physical and biochemical signals to establish epidermal cell polarity and maintain the hierarchical structure and function of skin tissue. However, how stem cell niches maintain tissue homeostasis and control ...[more]

PMID: 35817779

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