Proteomics

Dataset Information

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Analysis of phosphorylation sites of ZmRR1 phosphorylated by ZmMAPK8


ABSTRACT: To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were reduced by DTT and alkylated by IAM (Iodoacetamide), followed by digestion with trypsin (pH 8.5) at 37°C for 12 h. The results were analyzed by LC-MS/MS

ORGANISM(S): Zea Mays

SUBMITTER: Shuhua Yang  

PROVIDER: PXD027378 | iProX | Fri Jul 16 00:00:00 BST 2021

REPOSITORIES: iProX

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Publications

Natural variation in a type-A response regulator confers maize chilling tolerance.

Zeng Rong R   Li Zhuoyang Z   Shi Yiting Y   Fu Diyi D   Yin Pan P   Cheng Jinkui J   Jiang Caifu C   Yang Shuhua S  

Nature communications 20210805 1


Maize (Zea mays L.) is a cold-sensitive species that often faces chilling stress, which adversely affects growth and reproduction. However, the genetic basis of low-temperature adaptation in maize remains unclear. Here, we demonstrate that natural variation in the type-A Response Regulator 1 (ZmRR1) gene leads to differences in chilling tolerance among maize inbred lines. Association analysis reveals that InDel-35 of ZmRR1, encoding a protein harboring a mitogen-activated protein kinase (MPK) ph  ...[more]

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