Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adenylyl cyclase 3 deficiency results in the dysfunction of blood-testis barrier during mouse spermiogenesis

ABSTRACT: Human infertility has become a medical and social health problem globally. Mice deficient in type 3 adenylyl cyclase (AC3), a key enzyme that synthesizes cyclic adenosine monophosphate (cAMP), develop male infertility but its underlying molecular mechanisms are still unknown. Here, we performed label-free quantitative (LFQ) proteomics study to identify testicular differentially expressed proteins (DEPs) and their respective biological processes.

ORGANISM(S): Mus Musculus

SUBMITTER: Zhenshan Wang

PROVIDER: PXD027382 | iProX | Mon Jul 19 00:00:00 BST 2021

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Adenylyl cyclase 3 deficiency results in dysfunction of blood-testis barrier during mouse spermiogenesis.

Chen Tingrong T Zhou Yanfen Y Liu Xinxia X Liu Yuxin Y Yuan Junkai J Wang Zhenshan Z

Theriogenology 20211218

Human infertility has become a global medical and social health problem. Mice deficient in type 3 adenylyl cyclase (AC3), a key enzyme that synthesizes cyclic adenosine monophosphate (cAMP), develop male infertility, although the underlying molecular mechanisms remain unknown. We performed a label-free quantitative (LFQ) proteomics analyses to identify testicular differentially expressed proteins (DEPs) and their respective biological processes. Furthermore, histological examination demonstrated ...[more]

PMID: 34953349

Similar Datasets

Gene expression in undifferentiated or cyclic adenosine monophosphate-exposed BeWo trophoblast cells

Project description:BeWo trophoblast cells differentiate in response to expsure to cyclic adenosine monophosphate (cAMP) analogs. Differentiation includes syncytialization (fusion) and hormonogenesis. The goal of this study was to globally determine transcripts differentially expressed in BeWo trophoblast cells following a 24-h exposure to 250 uM 8-bromo-cAMP. 3 replicates undifferentiated BeWo trophoblast cells; and 3 replicates BeWo trophoblast cells treated with 250 uM 8-Br-cAMP for 24 h.

2015-08-01 | E-GEOD-66840 | biostudies-arrayexpress

Gene expression in undifferentiated or cyclic adenosine monophosphate-exposed BeWo trophoblast cells

2015-08-01 | GSE66840 | GEO

Dapagliflozin ameliorates diabetes-induced spermatogenic dysfunction by modulating the adenosine metabolism along the gut microbiota-testis axis

Project description:Male infertility is one of the most common complications of diabetes mellitus (DM). Dapagliflozin is widely used to manage the type II DM. This study aimed to assess the dapagliflozin’s effects on the spermatogenesis by administering either dapagliflozin (Dapa) or a vehicle (db) to diabetic male db/db mice, and using littermate male db/m mice as the control group (Con). We further performed the integrative analyses of the cecal shotgun metagenomics, cecal/plasmatic/testicular metabolomics, and testicular proteomics. We found that dapagliflozin treatment significantly alleviated the diabetes-induced spermatogenic dysfunction by improving sperm quality, including the sperm concentration and the sperm motility. The overall microbial composition was reshaped in Dapa mice and 13 species (such as Lachnospiraceae bacterium 3-1) were regarded as potential beneficial bacteria. Metabolites exhibited modified profiles, in which adenosine, cAMP, and 2’-deoxyinosine being notably altered in the cecum, plasma, and testis, respectively. Testicular protein expression patterns were similar between the Dapa and Con mice. In vivo results indicated that compared with db group, dapagliflozin treatment alleviated apoptosis and oxidative stress in testis tissues by down-regulating 2’-deoxyinosine. This was further validated by in vitro experiments using GC-2 cells. Our findings support the potential use of Dapa to prevent the diabetes-induced impaired sperm quality and to treat diabetic male infertility.

2024-01-04 | PXD034622 | Pride

2′ ,3′-cAMP treatment mimics abiotic stress response

Project description:For many years, 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP), a positional isomer of the second messenger 3′,5′-cAMP, has not received enough attention. Recent studies have reported that 2′,3′-cAMP exists in plants and might be involved in stress signaling because 1) its level increases upon wounding and 2) it was shown to participate in stress granule formation. Although 2′,3′-cAMP is known as RNA-degradation product, the effect of its accumulation in the cell remains unknown. Here, we unprecedentedly evaluate responses at the transcriptome, metabolome, and proteome levels to the accumulation of 2′,3′-cAMP in Arabidopsis plants. Data revealed that 2′,3′-cAMP is metabolized into adenosine, suggesting that a well-known cyclic nucleotide - adenosine pathway from human cells might exist also in plants. However, the control transcriptomic data indicated that, despite fewer overlaps, responses to 2′,3′-cAMP and to adenosine differ. Analysis of the transcriptome and proteome in response to 2′,3′-cAMP showed similar changes, known as signatures for abiotic stress response. This is further supported by the fact that adding to the role in facilitating of stress granules (SGs) formation, 2′,3′-cAMP induces changes at the level of proteins known as key components of SGs and can also induce movement of processing bodies (PBs) in the cell.

2022-05-19 | PXD028365 | Pride

Differentiation of human monocytes to macrophages in the presence of GM-CSF +/- forskolin (FSK)

Project description:Macrophages are central mediators of the innate immune system that can be differentiated from monocytes upon exposure to cytokines. While increased cyclic adenosine monophosphate (cAMP) levels inhibit many lipopolysaccharide (LPS) -elicited macrophage inflammatory responses, the effects of elevated cAMP on macrophage differentiation are not well understood. We have differentiated monocytes to macrophages in the presence of GM-CSF or GM-CSF + FSK to elevate cAMP levels and determine its effects on differentiation. Microarray analysis was performed separately on samples from 3 human donors: 3 control and 3 forskolin-treated samples.

2010-06-20 | E-GEOD-18654 | biostudies-arrayexpress

Differentiation of human monocytes to macrophages in the presence of GM-CSF +/- forskolin (FSK)

2009-10-22 | GSE18654 | GEO

Therapeutic melanoma inhibition by local micelle-mediated cyclic nucleotide repression

Project description:The acidic tumor microenvironment in melanoma drives immune evasion by up-regulating cyclic adenosine monophosphate (cAMP) in tumor-infiltrating monocytes..Melanoma growth can be suppressed by releasing non-toxic concentrations of an adenylate cyclase (AC) inhibitor from polypept(o)id micelles at the tumor site in an immune cell-dependent manner.

2021-08-19 | GSE166028 | GEO

Transcriptomic analysis of Arthrobacter sp. CGMCC 3584 responding to pde gene knockout

Project description:Arthrobacter sp. CGMCC 3584 are able to produce high yields of extracellular cyclic adenosine monophosphate (cAMP), which plays a vital role in the field of treatment of disease and animal food, during aerobic fermentation. Comparative transcriptomic analysis revealed that arpde inactivation had two major effects on metabolism: inhibition of glycolysis, PP pathway, and amino acid metabolism; promotion of the purine metabolism and carbon flux from the precursor PRPP, which benefited cAMP production.

2020-01-01 | GSE134961 | GEO

3’,5’-cAMP affects actin cytoskeleton dynamics by direct binding to actin.

Project description:Cyclic adenosine monophosphate (3’,5’-cAMP) is a well-characterized and evolutionary conserved second messenger. In contrast to the animal cells, however, the role of 3’,5’-cAMP in plants remains enigmatic. With the exception of cyclic nucleotide-gated ion channels, no plant 3’,5’-cAMP interactors have been reported to date. To address the existing gap, we resorted to thermal proteome profiling (TPP) as novel biochemical approach for the identification of protein binders of a small-molecule of choice. Based on the significant change in the thermal stability caused by 3’,5’-cAMP, we report a list of 51 putative 3’,5’-cAMP protein interactors, of which we focused on actin isovatriant ACT2. Most importantly we could demonstrate that 3’,5’-cAMP binding increases the rate of actin polymerization, resulting in longer actin filaments. Moreover, 3’,5’-cAMP treatment affected actin bundling, resulting in thicker actin bundles, as observed in the Arabidopsis hypocotyl cells. In line, with the obtained results 3’,5’-cAMP supplementation resulted in partial rescue of the short hypocotyl phenotype of the act2act7 Arabidopsis mutant, and 3’,5’-cAMP treatment suppressed actin depolymerization induced by Latrunculin B. Finally, 3’,5’-cAMP effects were independent from its degradation to adenosine, and true for nanomolar concentrations of 3’,5’-cAMP reported for the plant cells. Considering evolutionary conservation of 3’,5’-cAMP and actin we expect that our findings will be relevant to other eukaryotic organisms, and as such demonstrate a novel and direct mechanism of 3’,5’-cAMP regulation of the actin cytoskeleton dynamics.

2023-06-02 | PXD019537 | Pride

Metabolite annotation of rat epididymis with High-resolution imaging mass spectrometry

Project description:Infertility or subfertility is a widespread problem with approximately 25% of couples seeking medical help. For about half the cases it lies in the male partner. Male infertility is caused by problems that affect sperm production, maturation and/or transport. In this study the role played by the epididymis in the post-testicular maturation of spermatozoa during its transit through this organ was investigated using FT-ICR imaging mass spectrometry of metabolites and the FDR-controlled metabolite annotation platform MetaSpace for high-resolution imaging mass spectrometry. This study together with MTBLS313 provides the accompanying data for FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry.

2018-05-17 | MTBLS58 | MetaboLights

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data