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PUMILIO Proteins in Colorectal Cancer: Tumor Growth Promoting Function and Potential as Therapeutic Targets

ABSTRACT: We employ Mass spectrum to investigate proteome of Pum1-Knockout, Pum2-Knockout and WT conditions in human colorectal cancer cell line Hct116. Overall design: In order to investigate whether Pum1 and Pum2 regulate their targets at their protein levels, we used Pum1-Knockout, Pum2-Knockout and WT Hct116 cell line to extract total protein for Mass spectrum.

ORGANISM(S): Homo Sapiens

SUBMITTER: Haifan Lin

PROVIDER: PXD027513 | iProX | Fri Jul 23 00:00:00 BST 2021

REPOSITORIES: iProX

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PUMILIO proteins promote colorectal cancer growth via suppressing p21.

Gong Yuanyuan Y Liu Zukai Z Yuan Yihang Y Yang Zhenzhen Z Zhang Jiawei J Lu Qin Q Wang Wei W Fang Chao C Lin Haifan H Liu Sanhong S

Nature communications 20220325 1

PUMILIO (PUM) proteins belong to the highly conserved PUF family post-transcriptional regulators involved in diverse biological processes. However, their function in carcinogenesis remains under-explored. Here, we report that Pum1 and Pum2 display increased expression in human colorectal cancer (CRC). Intestine-specific knockout of Pum1 and Pum2 in mice significantly inhibits the progression of colitis-associated cancer in the AOM/DSS model. Knockout or knockdown of Pum1 and/or Pum2 in human CRC ...[more]

PMID: 35338151

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