Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of phenanthrene transfer proteins in xylem sap

ABSTRACT: The differentially expressed proteins in wheat xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.

ORGANISM(S): Triticum Aestivum

SUBMITTER: Xinhua Zhan

PROVIDER: PXD028208 | iProX | Wed Sep 15 00:00:00 BST 2021

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Mechanistic insights into phenanthrene acropetal translocation via wheat xylem: Separation and identification of transfer proteins.

Zeng Nengde N Zhu Yuting Y Gu Suodi S Wang Dongru D Chen Ruonan R Feng Qiurun Q Zhan Xinhua X Gardea-Torresdey Jorge L JL

The Science of the total environment 20220514 Pt 1

Polycyclic aromatic hydrocarbons (PAHs) have the potential to cause cancer, teratogenicity, and mutagenesis in humans. Long-term plant safe production relies on how PAHs are transported and coordinated across organs. However, the acropetal transfer mechanism of PAHs in staple crop stems, particularly in xylem, a critical path, is unknown. Herein, we first confirmed the presence of specific interaction between the proteins and phenanthrene by employing the magnetic phenanthrene-bound bead immunoa ...[more]

PMID: 35577096

Similar Datasets

Expression analysis of phenanthrene transfer proteins

Project description:The differentially expressed proteins in wheat xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.

2021-09-01 | PXD028203 | iProX

Identification of phenanthrene transfer proteins in soybeanxylem sap

Project description:The differentially expressed proteins in soybean xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.

2023-02-18 | PXD040258 | iProX

Identification of maize xylem sap

Project description:The differentially expressed proteins in maize xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05).

2023-02-18 | PXD040256 | iProX

Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress

Project description:Fusarium oxysporum f. sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change >=2 fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and ten of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions.

2016-01-26 | PXD003378 | Pride

microRNA data involved in T3 treated Japanese flounder (Paralichthys olivaceus)

Project description:After comparing albinism rate, T3 treatment and control larvae of 42 DAH were chosen for next high-throughput sequencing analyses of miRNA. A total of 337 miRNAs were identified via miRNA-seq and 12 miRNAs exhibited significantly differential expression patterns in D42_T3 vs D42_Con (TPM > 10, fold change ≥ 1.5 or ≤ 0.67 and q ≤ 0.05). These differentially expressed miRNAs targeted 3658 putative genes, further enriching 10 GO terms (q < 0.05).

2019-12-31 | GSE104919 | GEO

microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the miRNAs involved in P. olivaceus albinism. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05).

2017-07-13 | GSE99342 | GEO

Clavibacter nebraskensis secretome analysis

Project description:The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.

2021-09-08 | PXD014510 | Pride

Xylem sap shotgun proteomics from Fe- and Mn-deficient and Mn-toxic tomato plants

Project description:The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.

2017-11-30 | PXD007517 | Pride

Xylem sap shotgun proteomics from Mn-excess tomato plants

Project description:The aim of this work was to study the effects of Mn excess on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high- throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.

2020-12-10 | PXD021973 | Pride

Multi‐omics analysis of xylem sap uncovers dynamic modulation of poplar defenses by ammonium and nitrate

Project description:Xylem sap is the major transport route for nutrients from roots to shoots. In the present study, we investigated how variations in nitrogen (N) nutrition affected the metabolome and proteome of xylem sap and the growth of the xylem endophyte Brennaria salicis, and we also report transcriptional re-wiring of leaf defenses in poplar (Populus × canescens). We supplied poplars with high, intermediate or low concentrations of ammonium or nitrate. We identified 288 unique proteins in xylem sap. Approximately 85% of the xylem sap proteins were shared among ammonium- and nitrate-supplied plants. The number of proteins increased with increasing N supply but the major functional categories (catabolic processes, cell wall-related enzymes, defense) were unaffected. Ammonium nutrition caused higher abundances of amino acids and carbohydrates, whereas nitrate caused higher malate levels in xylem sap. Pipecolic acid and N-hydroxy-pipecolic acid increased, whereas salicylic acid and jasmonoyl-isoleucine decreased, with increasing N nutrition. Untargeted metabolome analyses revealed 2179 features in xylem sap, of which 863 were differentially affected by N treatments. We identified 124 metabolites, mainly from specialized metabolism of the groups of salicinoids, phenylpropanoids, phenolics, flavonoids, and benzoates. Their abundances increased with decreasing N, except coumarins. Brennaria salicis growth was reduced in nutrient-supplemented xylem sap of low- and high- NO3 - -fed plants compared to that of NH4 + -fed plants. The drastic changes in xylem sap composition caused massive changes in the transcriptional landscape of leaves and recruited defenses related to systemic acquired and induced systemic resistance. Our study uncovers unexpected complexity and variability of xylem composition with consequences for plant defenses.

2022-07-04 | MTBLS2305 | MetaboLights

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data