Dataset Information

Proteomics of normal and atretic follicles in geese

ABSTRACT: Follicular atresia is an important factor affecting reproductive performance. In birds, less than 5% of follicles could complete their development process and mature ovulation, while the other 95% become atretic follicles and eventually degraded3. Yangzhou geese are famous for their high egg production, however, the average number of eggs per goose is 40-80 and the peak laying rate is 40%-50%12. As an important meat source of poultry, the low egg production of goose has seriously affected the production cost and industrialization development. How to avoid follicular atresia and promote its normal development could help to improve egg production. Therefore, understanding the mechanism of follicular atresia in geese is of great significance for improving egg production.Isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) between normal and atretic follicles.

ORGANISM(S): Anser Canagica

SUBMITTER: Xingyong Chen

PROVIDER: PXD028836 | iProX | Wed Sep 29 00:00:00 BST 2021

REPOSITORIES: iProX

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Publications

Integrated transcriptome and proteome revealed that the declined expression of cell cycle-related genes associated with follicular atresia in geese.

Yang Wanli W Chen Xingyong X Liu Zhengquan Z Zhao Yutong Y Chen Yufei Y Geng Zhaoyu Z

BMC genomics 20230116 1

<h4>Background</h4>Geese exhibit relatively low reproductive performance, and follicular atresia is an important factor that restricts the egg production of geese. Systematic analysis of the regulation of follicle atresia in geese through transcriptome and proteome levels could provide meaningful information on clarifying the mechanism of follicle atresia in poultry.<h4>Result</h4>The granulosa cell layer was loose, disintegrated and showed apoptosis in atretic follicles and remained intact in n ...[more]

PMID: 36647001

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Project description:Molecular profiling of the eggs is an excellent approach aiming at understanding biological processes and mechanisms conditioning developmental competence in finfishes and, consequently, reproductive fitness. Despite many efforts, it is still unclear what is the specific role of transcriptome and proteome in determination of egg quality in Teleostei fishes. The aim of this study was to perform integrated transcriptomic-proteomic analysis of eggs of pikeperch – a commercially relevant freshwater fish species. Additionally, comparative analysis of transcriptome and proteome in eggs characterized by either high or low egg quality has been performed, in order to identify mechanisms leading to early embryonic lethality in pikeperch. Our study provides a novel insight into the understanding of the role of maternally-derived molecular cargo in finfishes. The data obtained sheds light on the importance of transcriptome in development of nervous system suggesting neurogenesis-related mRNAs as a very important, non-genetic inheritance factor. Proteomic analysis highlights specific role of proteins in the immune response in ovulated eggs. Integrated analysis brings attention to the galactose-specific lectin nattectin gene and protein as the frontline defense molecule for the egg and developing embryo. The molecular analysis of egg developmental competence emphasizes post-vitellogenic processes (final oocyte maturation and ovulation) as the ones potentially compromising transcriptomic profile, but not affecting proteomic cargo. It highlights the need for careful reconsideration of the commercial reproductive protocols as the very strong modulators of egg quality and their molecular profile. Considering the mechanisms driving these alterations as well as consequences stemming from the differential abundance of the transcripts are still to be explored, the candidate quality markers, first time provided for pikeperch along with the current study, are creating valuable resource for further studies.

Dataset Information

Proteomics of normal and atretic follicles in geese

Publications

Integrated transcriptome and proteome revealed that the declined expression of cell cycle-related genes associated with follicular atresia in geese.

Similar Datasets

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