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The influence of different Matrigel dissolving methods on proteome research of human derived organoids

ABSTRACT: Here we investigated the influence of different Matrigel dissolving methods on organoid proteomics.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Jianmin Wu

PROVIDER: PXD029173 | iProX | Tue Oct 12 00:00:00 BST 2021

REPOSITORIES: iProX

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In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.

Wang Man M Yu Huan H Zhang Ting T Cao Lihua L Du Yang Y Xie Yuhao Y Ji Jiafu J Wu Jianmin J

Molecular & cellular proteomics : MCP 20211203 1

Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation. However, organoids are usually cultured in murine Matrigel (served as scaffolds and matrix), which brings an issue to separate organoids from Matrigel. Because of the complex compositions of Matrigel and thousands of identical pep ...[more]

PMID: 34871808

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