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Proteomics of mouse ependymal cilia

ABSTRACT: Two 75-cm2 flasks of ependymal cells derived from ten E18.5 C57BL/6J mouse embryos at the day 10 post serum starvation were harvested and motile cilia were collected for mass spectrometry analysis.

ORGANISM(S): Mus Musculus

SUBMITTER: Xueliang Zhu

PROVIDER: PXD029174 | iProX | Mon Oct 18 00:00:00 BST 2021

REPOSITORIES: iProX

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Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Hao Kai K Chen Yawen Y Yan Xiumin X Zhu Xueliang X

Nature communications 20211130 1

Cilia are microtubule-based hair-like organelles propelling locomotion and extracellular liquid flow or sensing environmental stimuli. As cilia are diffusion barrier-gated subcellular compartments, their protein components are thought to come from the cell body through intraflagellar transport or diffusion. Here we show that cilia locally synthesize proteins to maintain their structure and functions. Multicilia of mouse ependymal cells are abundant in ribosomal proteins, translation initiation f ...[more]

PMID: 34848703

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