Dataset Information

Listeria monocytogenes responded to dehydrocorydaline

ABSTRACT: Listeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified and characterized a novel antibacterial agent, dehydrocorydaline, in Corydalis turschaninovii rhizome using HPLC-LTQ-Orbitrap-HRMS and its antibacterial effect with lowest MIC (1 mg/mL) and MBC (2 mg/mL) values. In addition, in vitro growth kinetic assay, cytoplasmic nucleic acid and protein leakage assay and observation of morphological changes in bacterial cells supported the strong antibacterial activity. Herein, quantitative proteomic analysis was performed to investigate the molecular mechanism of antibacterial action of dehydrocorydaline against L. monocytogenes. Taking together with proteomic results, we found that dehydrocorydaline influenced on the global proteomic alteration of L. monocytogenesis associated with carbohydrate metabolism, energy metabolism, cell wall synthesis, and bacterial motility, suggesting multiple target sites of dehydrocorydaline to inactivate bacterial growth. This study demonstrated that dehydrocorydaline has potential as a natural and effective antibacterial agent with multi-target sites in pathogenic bacteria, and provides the basis for development of a new class of antibacterial agent.

ORGANISM(S): Listeria Monocytogenes Egd Listeria Monocytogenes Egd-e

SUBMITTER: Zhongquan Sui

PROVIDER: PXD029222 | iProX | Tue Oct 19 00:00:00 BST 2021

REPOSITORIES: iProX

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Publications

Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes.

Kim Gowoon G Xu Yijuan Y Zhang Jiarong J Sui Zhongquan Z Corke Harold H

Frontiers in microbiology 20220111

Listeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified and characterized a novel antibacterial agent, dehydrocorydaline, in the Corydalis turschaninovii rhizome using HPLC-LTQ-Orbitrap-HRMS, and its antibacterial effect with lowest MIC (1 mg/ ...[more]

PMID: 35087499

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Project description:Milk, renowned for its nutritional value, harbours bioactive compounds, including lactoferrin (Lf) [1], immunoglobulins, and functional proteins suggesting broader functionalities beyond nutrition. Lf, an iron glycoprotein, exhibits antimicrobial properties against bacteria, fungi, and viruses."In"this st"dy, Immune Powder (a functional dairy formulation) and Fractionated Milk Protein (FMP; a blend of bioactive proteins isolated from skim milk) containing Lf, zinc, and immunoglobulins produced by Ausnutria Australia Dairy Pty Ltd were evaluated for their broad spectrum pharmacological activity. In particular, the study aims to investigate the antibacterial (against pathogenic Escherichia coli), prebiotic (promoting Lactobacillus delbrueckii growth), anti-inflammatory (against RAW264.7 macrophages), and antiviral (against human coronavirus 229E, HCoV-229E cultured on MCR-5 cells) effects of the formulations. In addition, the impact of simulated gastric digestion on the efficacy of the formulations was explored. LCMS-based proteomics analysis was implemented to unveil cellular and molecular mechanisms underlying antiviral activity. The Immune Powder demonstrated antibacterial activity against E. coli (up to 99.74 ± 11.47% inhibition at 62.5 mg/mL), coupled with prebiotic action (10.84 ± 2.2 viability fold change at 125 mg/mL), albeit diminished post-digestion (p <0.01). The Immune Powder effectively mitigated inflammation by reducing NO production, with efficacy declining post-digestion (p < 0.0001). The potential antiviral activity of Immune Powder against HCoV-229E also shown an increase of 2.62 ± 0.42 in cell viability when treated at 500 µg/mL. Similarly, FMP exhibited antibacterial potency pre-digestion at high concentration (95.56 ± 1.23% inhibition at 125 mg/mL), and post-digestion at lower doses (61.82 ± 5.58% inhibition at 3906.25 µg/mL). FMP also shown enhanced prebiotic activity post-digestion (p < 0.0001), anti-inflammation pre-digestion, and significant antiviral activity against HCoV-229E (1.72 ± 0.52 cell viability fold change). The proteomics study suggested both formulas shared similar antiviral mechanism by inhibiting scavenger receptor binding and ECM interaction.

Dataset Information

Listeria monocytogenes responded to dehydrocorydaline

Publications

Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From <i>Corydalis turtschaninovii</i> Bess. Against <i>Listeria monocytogenes</i>.

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