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Proteome analysis of outer membrane vesicles from a highly virulent strain of Haemophilus parasuis

ABSTRACT: In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using LC-MS/MS identification and bio-information analysis, we identified 588 different proteins associated with OMVs. And we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism.

ORGANISM(S): Glaesserella Parasuis

SUBMITTER: Kunli Zhang

PROVIDER: PXD029451 | iProX | Mon Nov 01 00:00:00 GMT 2021

REPOSITORIES: iProX

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Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis.

Zhang Kunli K Chu Pinpin P Song Shuai S Yang Dongxia D Bian Zhibiao Z Li Yan Y Gou Hongchao H Jiang Zhiyong Z Cai Rujian R Li Chunling C

Frontiers in veterinary science 20211126

Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is a ...[more]

PMID: 34901247

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