Dataset Information

Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars

ABSTRACT: In this study, a comparative proteomics analysis was performed through the iTRAQ technique and the activity of defense-related enzymes was assessed. We evaluated the expression of differentially expressed proteins and defense response in two flue-cured tobacco cultivars, i.e., K326 (resistant) and Hongda (susceptible) with contrasting resistance to black shank.

ORGANISM(S): Nicotiana Tabacum

SUBMITTER: Zhengxiong Zhao

PROVIDER: PXD030872 | iProX | Mon Jan 10 00:00:00 GMT 2022

REPOSITORIES: iProX

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Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars.

Song Ruifang R Tan Yujiao Y Ahmed Waqar W Zhou Guisu G Zhao Zhengxiong Z

BMC microbiology 20220423 1

<h4>Background</h4>Black shank disease caused by Phytophthora nicotianae is a serious threat to flue-cured tobacco production. Whole-plant resistance is characterized by the expression of a number of pathogenesis-related proteins, genes, and the activity of different defense-related enzymes. In this study, we investigated the activity of defense-related enzymes and expression of differentially expressed proteins through the iTRAQ technique across two flue-cured tobacco cultivars, i.e., K326 and ...[more]

PMID: 35461247

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Project description:Although Pseudomonas aeruginosa is an opportunistic pathogen that does not often naturally infect alternate hosts such as plants, the plant-P. aeruginosa model has become a widely recognized system for identifying new virulence determinants and studying pathogenesis of this organism. Here we examine how both host factors and P. aeruginosa PAO1 gene expression are affected in planta after infiltration into incompatible and compatible cultivars of tobacco (Nicotiana tabacum L.) Nicotiana tabacum has a resistance gene (N) against tobacco mosaic virus; and although resistance to PAO1 infection correlated to the presence of a dominant N-gene, our data suggests that it is not a factor in resistance against Pseudomonas. We did observe that the resistant tobacco cultivar had higher basal levels of salicylic acid, and a stronger salicylic acid response upon infiltration of PAO1. Salicylic acid acts as a signal to activate defense responses in plants, limiting the spread of the pathogen and preventng access to nutrients. It has also been shown to have direct virulence modulating effects on P. aeruginosa. We also examined host effects on the pathogen by analyzing global gene expression profiles of bacteria removed from the intracellular fluid of the two plant hosts. We discovered that the availability of micronutrients, particularly sulfate and Pi, are important factors in in planta pathogenesis, and that the amounts of these nutrients made available to the bacteria may in turn have an effect on virulence gene expression. Indeed, there are several reports suggesting that P. aeruginosa virulence is influenced in mammalian hosts by the availability of iron and by levels of O2. Experiment Overall Design: Pseudomonas aeruginosa cells were removed from leaf tissue 24 hours post-infiltration and RNA was directly extracted from these cells. Comparisons are between cells removed from a susceptible cultivar of Nicotiana tabacum (cv. Samsun) and a resistant cultivar (cv. Xanthi). Three biological replicates per cultivar were analyzed.

Dataset Information

Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars

Publications

Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars.

Similar Datasets

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