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Nuclear condensation of CDYL links histone crotonylation and cystogenesis in autosomal dominant polycystic kidney disease

ABSTRACT: We identify the Kcr sites on histones that are targeted by CDYL in ADPKD cells (WT9-12 cells). We chose to knock out CDYL in WT 9-12 cells (CDYL KO) through CRISPR/Cas9-mediated gene editing to increase the overall levels of histone Kcr. We quantified Kcr modifications on different histone residues in CDYL KO versus parental WT 9-12 cells (control) using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS)-based proteomics analysis

ORGANISM(S): Homo Sapiens

SUBMITTER: Yupeng Chen

PROVIDER: PXD031029 | iProX | Mon Jan 17 00:00:00 GMT 2022

REPOSITORIES: iProX

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Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Dang Lin L Cao Xinyi X Zhang Tianye T Sun Yongzhan Y Tian Shanshan S Gong Tianyu T Xiong Hui H Cao Peipei P Li Yuhao Y Yu Shengqiang S Yang Li L Zhang Lirong L Liu Tong T Zhang Kai K Liang Jing J Chen Yupeng Y

Journal of the American Society of Nephrology : JASN 20220802 9

<h4>Background</h4>Emerging evidence indicates that epigenetic modulation of gene expression plays a key role in the progression of autosomal dominant polycystic kidney disease (ADPKD). However, the molecular basis for how the altered epigenome modulates transcriptional responses, and thereby disease progression in ADPKD, remains largely unknown.<h4>Methods</h4>Kidneys from control and ADPKD mice were examined for the expression of CDYL and histone acylations. CDYL expression and its correlation ...[more]

PMID: 35918147

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Nuclear condensation of CDYL links histone crotonylation and cystogenesis in autosomal dominant polycystic kidney disease [ChIP-Seq]

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