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Protein glycosylation changes during systemic acquired resistance in Arabidopsis thaliana

ABSTRACT: N-glycosylation is an important post-translational modification of proteins in all eukaryotes and involved in a number of diseases in mammalian systems. However, little is known about the role of protein N-glycosylation in plant defense responses to pathogen invasion. In the present study, we first identified glycoproteins related to systemic acquired resistance (SAR) in an Arabidopsis thaliana model using glycoproteomics platform based on high-resolution mass spectrometry. In total, 427 glycosylate sites corresponding to 391 glycopeptides and 273 unique glycoproteins were identified. A total of 65 significantly changed glycoproteins with 80 N-glycosylation were detected in systemic leaves of SAR-induced plants, including numerous GDSL-like lipases, thioglucoside glucohydrolases, kinases and glycosidases. A variety of significantly changed glycoproteins were involved in stomatal movement, and stomata aperture measurements further confirmed that stomata movement were regulated in systemic leaves of SAR-induced plants, suggesting that these proteins may be functionally involved in systemic stomatal immunity through glycosylation or deglycosylation. Functional enrichment analysis reveals that the significantly changed glycoproteins were mainly involved in N-glycan biosynthesis and degradation, phenylpropanoid biosynthesis, cutin and wax biosynthesis, plant-pathogen interactions. Comparative analysis of glycoproteomics data with proteomics and transcriptomics data suggest that these significantly changed glycoproteins were mainly regulated by post-translational modification during SAR. This study provides substantial insight into the role of protein glycosylation in SAR.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Qingfeng Zhou

PROVIDER: PXD031184 | iProX | Mon Jan 24 00:00:00 GMT 2022

REPOSITORIES: iProX

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Project description:In the absence of adaptive immunity displayed by animals, plants respond locally to biotic challenge via inducible basal defense networks activated through recognition and response toconserved pathogen associated molecular patterns (PAMPs). In addition, immunity can be induced in tissues remote from infection sites via systemic acquired resistance (SAR), initiated following gene-for-gene recognition between plant resistance proteins and microbial effectors.The nature of the mobile signal and remotely activated networks responsible for establishing SAR remain unclear. Here we show that despite the absence of PAMP contact, systemically responding leaves rapidly activate a SAR transcriptional signature with strong similarity to local basal defense. Jasmonates have previously been implicated in systemic signalling in response to wounding and plant herbivory but not SAR. We present several lines of evidence that suggest jasmonates may also be central to SAR. Jasmonic acid (JA) rapidly accumulates in phloem exudates of leaves challenged with an avirulent strain of Pseudomonas syringae. In systemically responding leaves transcripts associated with jasmonate biosynthesis are upregulated and JA increases transiently. SAR can be mimicked by foliar JA application and is abrogated in mutants impaired in jasmonate synthesis or response. We conclude that, jasmonate signalling appears to mediate long-distance information transmission. Moreover, the systemic transcriptional response shares extraordinary overlap with local herbivory and wounding responses, indicating that jasmonates may be central to an evolutionarily conserved signalling network, which decodes multiple abiotic and biotic stress signals. Experimenter name: William Truman Experimenter phone: +44 (0)1392 263789 Experimenter fax: +44 (0)1392 263434 Experimenter address: School of Biosciences Experimenter address: Geoffrey Pope Building Experimenter address: Stocker Road Experimenter address: Exeter Experimenter address: Devon Experimenter zip/postal_code: EX4 4QD Experimenter country: UK Keywords: infection

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Protein glycosylation changes during systemic acquired resistance in Arabidopsis thaliana

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