Dataset Information

Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus

ABSTRACT: Assassin bugs are one of the diversified groups of venomous predatory insects. Their venom is secreted from three different lumens of salivary gland and can lead to paralysis, lethality and liquidation of the prey, respectively. Nevertheless, the venom components responsible for these effects are not clear. In this study, we use transcriptomics and proteomics to determine the effective salivary protein components in the separate lumen of salivary gland from the assassin bug Platymeris biguttatus, and to conduct toxicological analysis on the function of the salivary gland compartments. Our study sheds light on the functional cooperation between different salivary gland lumens of assassin bugs, and will further the understanding of physiological adaptations of venom-based predation and defense in venomous insects

ORGANISM(S): Platymeris Biguttatus

SUBMITTER: Hu Li

PROVIDER: PXD031473 | iProX | Sun Feb 06 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, <i>Platymeris biguttatus</i>.

Gao Fanding F Tian Li L Li Xinyu X Zhang Yinqiao Y Wang Tianfang T Ma Ling L Song Fan F Cai Wanzhi W Li Hu H

Biology 20220317 3

Assassin bugs use their salivary venoms for various purposes, including defense, prey paralyzation, and extra-oral digestion, but the mechanisms underlying the functional complexity of the venom remain largely unclear. Since venom glands are composed of several chambers, it is suggested that individual chambers may be specialized to produce chemically distinct venoms to exert different functions. The current study assesses this hypothesis by performing toxicity assays and transcriptomic and prot ...[more]

PMID: 35336837

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Project description:Background The generalist dipteran pupal parasitoid Nasonia vitripennis injects 79 venom peptides into the host before egg laying. This venom induces several important changes in the host, including developmental arrest, immunosuppression, and alterations to normal metabolism. It is hoped that diverse and potent bioactivities of N. vitripennis venom provide an opportunity for the design of novel acting drugs. However, currently very little is known about the individual functions of N. vitripennis venom peptides and less than half can be bioinformatically annotated. The paucity of annotation information complicates the design of studies that seek to better understand the potential mechanisms underlying the envenomation response. Although the RNA interference system of N. vitripennis provides an opportunity to functionally characterise venom encoding genes, with 79 candidates this represents a daunting task. For this reason we were interested in determining the expression levels of venom encoding genes in the venom gland, such that this information could be used to rank candidate venoms. To do this we carried out deep sequencing of the transcriptome of the venom gland and neighbouring ovary tissue and used RNA-seq to measure expression from the 79 venom encoding genes. The generation of a specific venom gland transcriptome dataset also provides further opportunities to investigate novel features of this highly specialised organ. Results High throughput sequencing and RNA-seq revealed that the highest expressed venom encoding gene in the venom gland was a serine protease called Nasvi2EG007167, which has previously been implicated in the apoptotic activity of N. vitripennis venom. As expected the RNA-seq confirmed that the N. vitripennis venom encoding genes are almost exclusively expressed in the venom gland relative to the neighbouring ovary tissue. Novel peptides appear to perform key roles in N. vitripennis venom function as only four of the highest 15 expressed venom encoding genes are bioinformatically annotationed. The high throughput sequencing data also provided evidence for the existence of an additional 471 novel genes in the Nasonia genome that are expressed in the venom gland and ovary. Finally, metagenomic analysis of venom gland transcripts identified viral transcripts that may play an important part in the N. vitripennis venom function. Conclusions The expression level information provided here for the 79 venom encoding genes provides an unbiased dataset that can be used by the N. vitripennis community to identify high value candidates for further functional characterisation. These candidates represent bioactive peptides that have value in drug development pipelines.

Dataset Information

Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus

Publications

Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, <i>Platymeris biguttatus</i>.

Similar Datasets

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