Dataset Information

Proteomic analysis of key organs reveal the significant roles of antioxidant enzymes and oxidative phosphorylation proteins in starved ticks Haemaphysalis longicornis Neumann

ABSTRACT: Haemaphysalis longicornis Neumann, a tick of public health and veterinary importance, spend the major part of their life cycle off-host, especially the adult host-seeking period. Thus, they have to contend with prolonged starvation (sometimes for an extended period of 1-2 years). Here, we investigated the underlying molecular mechanism of tick starvation endurance in the salivary gland, midgut, ovary, and Malpighian tubule of starved H. longicornis ticks using the data-independent acquisition (DIA) quantitative proteomic approach to study the proteome changes. “Ribosome” was the most enriched KEGG pathway with the participation of more than thirty differentially expressed ribosomal proteins, indicating ribosomal stress. Essential synthases such as glutamate synthase, citrate synthase, ATP synthase, and ATP-citrate synthase were upregulated probably due to increased proteolysis and amino acid catabolism during starvation. The upregulation of succinate dehydrogenase, ATP synthase, cytochrome c oxidase, and ADP/ATP translocase closely fits with an increased oxidative phosphorylation function during starvation. The differential expression of superoxide dismutase, glutathione reductase, glutathione S-transferase, thioredoxin, thioredoxin reductase, and peroxiredoxin indicated fasting-induced oxidative stress. The upregulation of heat shock proteins could imply the activation of a protective mechanism that checks excessive protein breakdown during starvation stress. The survival of the ticks reduced after silencing of target genes via RNAi but was not significant relative to GFP-dsRNA injected ticks. The results of this study could provide useful information about the vulnerabilities of ticks that could aid in tick control efforts.

ORGANISM(S): Haemaphysalis Longicornis

SUBMITTER: Hui Wang

PROVIDER: PXD031596 | iProX | Thu Feb 10 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Proteomic changes in various organs of Haemaphysalis longicornis under long-term starvation.

Wang Ningmei N Wang Han H Ji Aimeng A Li Ning N Chang Guomin G Liu Jingze J Agwunobi Desmond O DO Wang Hui H

PLoS neglected tropical diseases 20220822 8

Haemaphysalis longicornis (Neumann), a tick of public health and veterinary importance, spend the major part of their life cycle off-host, especially the adult host-seeking period. Thus, they have to contend with prolonged starvation. Here, we investigated the underlying molecular mechanism of tick starvation endurance in the salivary glands, midguts, ovaries, and Malpighian tubules of starved H. longicornis ticks using the data-independent acquisition quantitative proteomic approach to study th ...[more]

PMID: 35994434

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Project description:Tick saliva contains many bioactive molecules that are involved in attachment to the host, blood feeding and transmission of pathogens. MicroRNAs (miRNAs) are a class of short non-coding RNAs with a length of 19-24 nucleotides. They act as regulators of gene expression by binding to their target mRNA at the post-transcriptional level and control a variety of cellular functions, including regulation of growth, metabolism, and development. The detection and characterizations of miRNAs from tick saliva may help explain the molecular mechanisms involved in the interaction between ticks, pathogens, and hosts. They may also contribute to the discovery of vaccines, which can control ticks and the pathogens they transmit. An RNA library was generated from the saliva of fed adult Haemaphysalis longicornis ticks, and it contained 17.4 million clean reads of 18-30 nucleotides. Overall, 319 known miRNAs and 1 novel miRNA were found. The 10 most abundantly expressed miRNAs present in tick saliva were miR-100_2, miR-315, miR-184_1, miR-100-5p_2, miR-5307, miR-184-3p_3, Let-7-5p_6, miR-71_5, miR-1-3p_6, and miR-10-5p_2. The miR-375, one of the abundantly expressed, was subjected to Quantitative Real-Time PCR analysis (qRT-PCR) in various tick developmental stages, as well as in different tissues isolated from adult ticks. The expression of miR-375 in different tick development stages was highest in unfed nymphs and lowest in the egg stage. In the tissues of adult ticks, miR-375 was most highly expressed in the salivary gland. To investigate the possible role of miR-375, Ant-375 was used to inhibit the miR-375. Treated group (Ant-375) had a reduced number of eggs (t(10)= 2.652, P=0.0242), eggs that were partially desiccated, and reduced egg hatchability (t(10)=2.272, P=0.044) compared to Ms-Ant and the non-injected control. This is the first study to investigate the miRNAs profile in tick saliva and the role of miR-375 in H. longicornis. The identification and characterization of miRNA in tick saliva may help to reveal the molecular mechanisms of interactions among ticks, pathogens, and hosts and suggest new vaccine strategies to control tick borne diseases.

Dataset Information

Proteomic analysis of key organs reveal the significant roles of antioxidant enzymes and oxidative phosphorylation proteins in starved ticks Haemaphysalis longicornis Neumann

Publications

Proteomic changes in various organs of Haemaphysalis longicornis under long-term starvation.

Similar Datasets

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