Proteomics

Dataset Information

0

Proteomic analysis of linear ubiquitination substrates based on NAEK-Ub probe in live cells

ABSTRACT: While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination, the none-specific binding of linear polyubiquitin chain, and the high background interferences arising from heterogeneous ubiquitin linkages (e.g. K48-, K63-). We developed a bioorthogonal linear ubiquitin probe by site-specific encoding of a norbornene amino acid (NAEK) on ubiquitin. This probe facilitates covalent labeling of linear ubiquitin substrates in live cells and enables selective enrichment and identification of linear-ubiquitin modified proteins. we applied this probe in the profiling of substrates of linear ubiquitination in HEK293T cells and GSCs.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaotao Duan  

PROVIDER: PXD031600 | iProX | Fri Feb 11 00:00:00 GMT 2022

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma.

Du Xianli X   Pang Jing J   Gu Bin B   Si Tian T   Chang Yan Y   Li Tianqi T   Wu Min M   Wang Zicheng Z   Wang Yuxia Y   Feng Jiannan J   Wu Ning N   Man Jianghong J   Li Huiyan H   Li Ailing A   Zhang Tong T   Wang Bo B   Duan Xiaotao X  

Nucleic acids research 20230201 3

While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination and the background interference arising from highly abundant ubiquitin linkages (e.g. K48- and K63-) and from the non-specific attachment of interfering proteins to the linear polyubiquitin chain. We developed a bio-orthogonal linear ubiq  ...[more]

Similar Datasets

Proteomics Simultaneous substrate and ubiquitin modification recognition by bispecific antibodies allows detection of ubiquitinated RIP1 and RIP2
2023-01-24 | MSV000091134 | MassIVE
Transcriptomics Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function
2018-02-08 | GSE107313 | GEO
Proteomics A novel antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
2020-11-30 | MSV000086537 | MassIVE
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042702 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD047455 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042698 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042674 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042700 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042697 | Pride
Proteomics Ubiquitin-specific proximity labeling for the identification of E3 ubiquitin ligase substrates
2024-03-20 | PXD042673 | Pride