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Quantitiave analysis of protein methylome reveals the cellular response to ionizing radiation

ABSTRACT: Protein methylation plays important roles in DNA damage signaling. To date, there is still a lack of global profiling of whole-cell methylation changes during the DNA damage response and repair. In this study, using HILIC affinity enrichment combined with MS analysis, we conducted a quantitative analysis of the methylated proteins in HEK293T cells in response to IR-induced DNA damage. In total, 235 distinct methylation sites responding to IR treatment were identified, and 38% of them were previously unknown. Multiple RNA-binding proteins were differentially methylated upon DNA damage stress. Furthermore, we identified 14 novel methylations in DNA damage response-related proteins. Moreover, we validated the function of PARP1 K23 methylation in repairing IR-induced DNA lesions.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yali Chen

PROVIDER: PXD031655 | iProX | Mon Feb 14 00:00:00 GMT 2022

REPOSITORIES: iProX

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Quantitative Analysis of the Protein Methylome Reveals PARP1 Methylation is involved in DNA Damage Response.

Wang Xinzhu X Mi Shaojie S Zhao Mingxin M Lu Chen C Jia Chenxi C Chen Yali Y

Frontiers in molecular biosciences 20220629

Protein methylation plays important roles in DNA damage response. To date, proteome-wide profiling of protein methylation upon DNA damage has been not reported yet. In this study, using HILIC affinity enrichment combined with MS analysis, we conducted a quantitative analysis of the methylated proteins in HEK293T cells in response to IR treatment. In total, 235 distinct methylation sites responding to IR treatment were identified, and 38% of them were previously unknown. Multiple RNA-binding prot ...[more]

PMID: 35847980

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