Dataset Information

Integrated Network Pharmacology and Proteomics to Reveal the Molecular Therapeutic Effects of Buyang Huanwu Decoction on Ischemic Stroke

ABSTRACT: Ischemic stroke (IS), as a common cerebrovascular disease, is characterized by high disability, mortality, recurrence, and complications. Studies have shown that Buyang Huanwu Decoction (BHD) can impact the treatment of acute cerebral infarction, subsequent recovery, and sequelae. However, the mechanism of action of BHD in IS treatment remains unclear. To this end, we applied a combined network pharmacology and proteomics approach to investigate the mechanism of action of BHD. First, the chemical components of BHD were revealed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS/MS). The molecular mechanism of BHD in treating IS was preliminarily elucidated through a network pharmacological approach. Subsequently, proteomic studies were used to explore the biomarkers of BHD in IS treatment

ORGANISM(S): Rattus Rattus

SUBMITTER: Lisheng Wang

PROVIDER: PXD032189 | iProX | Fri Mar 11 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS.

He Yingying Y Zheng Haozhen H Zhong Lanying L Zhong Nijun N Wen Guiqing G Wang Lisheng L Zhang Ying Y

Drug design, development and therapy 20211230

<h4>Objective</h4>The Chinese medicine Huangqi Guizhi Wuwu Decoction (HGWD) has been reported to improve the clinical symptoms and restore nerve function after ischemic stroke; however, its active ingredients are not well-determined. Therefore, this study aimed to investigate the bioactive compounds of HGWD and explore the possible mechanism of action.<h4>Methods</h4>The methods, including live HT22 cells, solid-phase extraction, and HPLC-MS/MS were utilized. The potential ingredients were ident ...[more]

PMID: 35002223

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Dataset Information

Integrated Network Pharmacology and Proteomics to Reveal the Molecular Therapeutic Effects of Buyang Huanwu Decoction on Ischemic Stroke

Publications

Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS.

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