Dataset Information

Proteomic raw data of wheat accessions Xinong291S and Xinong291

ABSTRACT: Raw proteomic data from three stages of anthers in wheat accessions Xinong291 and Xinong291S

ORGANISM(S): Triticum Aestivum

SUBMITTER: Rongqi Liang

PROVIDER: PXD032250 | iProX | Fri Mar 11 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (<i>Triticum aestivum</i> L.).

Tian Shuai S Zhang Minghu M Li Jinghui J Wen Shaozhe S Bi Chan C Zhao Huanhuan H Wei Chaoxiong C Chen Zelin Z Yu Jiazheng J Shi Xintian X Liang Rongqi R Xie Chaojie C Li Baoyun B Sun Qixin Q Zhang Yufeng Y You Mingshan M

Frontiers in plant science 20211207

Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and is closely related to baking quality. In this study, a total of 15 quantitative trait locus (QTL) for sodium dodecyl sulfate (SDS)-sedimentation volume (SSV) were identified by using a high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with a doubled haploid (DH) population derived from the cross between Non-gda3753 (ND375 ...[more]

PMID: 34950162

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Project description:Our understanding of the mechanisms that govern the cellular process of meiosis is limited in higher plants with polyploid genomes. Bread wheat is an allohexaploid that behaves as a diploid during meiosis. Chromosome pairing is restricted to homologous chromosomes despite the presence of homoeologues in the nucleus. The importance of wheat as a crop and the extensive use of wild wheat relatives in breeding programs has prompted many years of cytogenetic and genetic research to develop an understanding of the control of chromosome pairing and recombination. The rapid advance of biochemical and molecular information on meiosis in model organisms such as yeast provides new opportunities to investigate the molecular basis of chromosome pairing control in wheat. However, building the link between the model and wheat requires points of data contact. We report here a large-scale transcriptomics study using the Affymetrix wheat GeneChipÂ® aimed at providing this link between wheat and model systems and at identifying early meiotic genes. Analysis of the microarray data identified 1,350 transcripts temporally-regulated during the early stages of meiosis. Expression profiles with annotated transcript functions including chromatin condensation, synaptonemal complex formation,recombination and fertility were identified. From the 1,350 transcripts, 30 displayed at least an eight-fold expression change between and including pre-meiosis and telophase II, with more than 50% of these having no similarities to known sequences in NCBI and TIGR databases. This resource is now available to support research into the molecular basis of pairing and recombination control in the complex polyploid, wheat. Experiment Overall Design: The seven stages collected were pre-meiosis (PM), leptotene to pachytene (LP), diplotene to anaphase I (DA), telophase I to telophase II (TT), tetrads (T), immature pollen (IP) and mature anthers (MAN). Immediately after determining the stage, the remaining two anthers from the floret were placed into liquid nitrogen. After collecting at least 25 staged anthers for each time point from several biological samples, anthers from the respective stages were pooled. Leaf material used in this study was collected from glasshouse-grown plants (six weeks) and total RNA isolated using TrizolÂ® (Gibco BRL, Australia) according to the manufacturerâs protocol. Experiment Overall Design: Twenty micrograms of aRNA of from each of the seven samples were fragmented for hybridization to each microarray. In total three technical replicates were conducted for each of the seven stages examined. Affymetrix GeneChipÂ® Wheat Genome Arrays were used for all samples. The arrays were hybridized and processed according to the manufacturerâs specifications. Experiment Overall Design: Normalization of the microarray data was conducted using RMA. The software package Acuity 4 (Molecular Devices, CA, USA) was then used to analyze the microarray data.

Dataset Information

Proteomic raw data of wheat accessions Xinong291S and Xinong291

Publications

Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (<i>Triticum aestivum</i> L.).

Similar Datasets

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