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Proximity tagging identified mitofusins stimulating mitochondria and peroxisomes clustering

ABSTRACT: we applied a proximity tagging system PUP-IT to PEX ubiquitin ligase to identify their proximal proteins. Surprisingly, we identified many mitochondrial proteins in the proximity of peroxisomes. We further validated the interaction between PEX and mitofusins (MFNs).

ORGANISM(S): Homo Sapiens

SUBMITTER: Min Zhuang

PROVIDER: PXD032661 | iProX | Sat Mar 19 00:00:00 GMT 2022

REPOSITORIES: iProX

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The MFN1 and MFN2 mitofusins promote clustering between mitochondria and peroxisomes.

Huo Yinbo Y Sun Weiping W Shi Tiezhu T Gao Song S Zhuang Min M

Communications biology 20220506 1

Mitochondria and peroxisomes are two types of functionally close-related organelles, and both play essential roles in lipid and ROS metabolism. However, how they physically interact with each other is not well understood. In this study, we apply the proximity labeling method with peroxisomal proteins and report that mitochondrial protein mitofusins (MFNs) are in proximity to peroxisomes. Overexpression of MFNs induces not only the mitochondria clustering but also the co-clustering of peroxisomes ...[more]

PMID: 35523862

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