Dataset Information

Proteomic Characterization of Extracted and Purified IgY Antibodies

ABSTRACT: An increasing demand for the development of immunoglobin Y (IgY) illustrates the necessity of the component analysis in the process of conduction and quality control. This study investigated the proteome changes during IgY extraction (polyethylene glycol precipitation) and purification (a human mycoplasma protein-based affinity chromatography) from hen egg yolk. After confirming the extraction efficiency and purity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, bottom-up label-free liquid chromatography tandem-mass spectrometry (LC-MS/MS) was performed with samples including fresh yolk, IgY extracted product and purified product (all samples in triplicates). A total of 348 proteins were identified, with 36 proteins deleted and 209 newly detected proteins in the final IgY preparation compared to the intact egg yolk. The significantly reduced proteins mainly included phosvitin, albumin and apolipoprotein B whereas the significantly increased proteins were mainly IgY-related proteins and peptidase S1 domain-containing protein. GO functional analysis showed that compared to the PEG precipitated product, the differentially expressed protein in the IgY preparation obtained after protein M purification mainly had ATPase activity and purine ribonucleoside triphosphate binding activity and was mainly involved in purine and nucleic acid metabolism. This study will inevitably fasten the commercial application of IgY antibodies and is of greater significance for promotion, development and approval for new antibody derived drug products.

ORGANISM(S): Gallus Gallus

SUBMITTER: Xiaoying Zhang

PROVIDER: PXD032865 | iProX | Mon Mar 28 00:00:00 BST 2022

REPOSITORIES: iProX

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Proteomic investigation and understanding on IgY purification and product development.

Zhang Xiaoying X Wu Rao R Chelliappan Brindha B

Poultry science 20230605 8

An increasing demand for the development of immunoglobin Y (IgY) illustrates the necessity of the component analysis in the process of conduction and quality control. This study investigated the proteomic changes in crude IgY extracts and purified IgY products obtained by sequential polyethylene glycol precipitation (PEG) of egg yolks followed by human mycoplasma protein-based affinity chromatography compared with intact egg yolks. After confirming the extraction efficiency and purity by sodium ...[more]

PMID: 37329629

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Project description:Background: Poultry eggs are a low cost, high protein nutrient package that can be consumed as part of a quality diet. However, consumption of poultry egg products has been historically contentious, which highlights the importance of investigating impacts of long-term egg consumption on metabolic health. Objective: Our study utilized the zebrafish Danio rerio, a newly-defined model of human metabolic health, to understand the metabolic consequence of consuming egg products in lieu of other well-described protein sources. Methods: Reference diets were formulated to contain multi-source protein with casein and fish protein hydrolysate (CON; control protein sources), the protein sources of which have been vetted in numerous reference diets. These proteins were then replaced in part with either whole egg (WE; protein and lipid source), egg white (EW; protein source), wheat gluten (WG; cereal protein source), or a high lipid content diet containing a multi-source protein with casein and fish protein hydrolysate (HFCON; isonitrogenous and isolipidic with the WE diet) in a 34-week trial. Daily feeding was initiated at early juvenile life stage and terminated at late reproductive adult stage. Results: The amino acid composition of control vs egg product diets did not vary substantially, although methionine and lysine were apparently limiting in fish fed WG. At termination, fish fed EW as the protein source had similar weight gain and body composition to those fed the CON diet. Blood glucose, both fasting and postprandial, did not differ among any dietary treatment. Assessment of the liver transcriptome using RNAseq revealed no differential gene expression between zebrafish fed CON or WE diets. Zebrafish fed WG had lower weight gain in males. Conclusions: Long-term consumption of egg products promoted metabolic health equal to that of historically-relevant proteins. These data support the value of egg products for maintaining long-term metabolic health in animal diets.

Dataset Information

Proteomic Characterization of Extracted and Purified IgY Antibodies

Publications

Proteomic investigation and understanding on IgY purification and product development.

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