The protein profiles of neural stem cells

ABSTRACT: Stemazole was reported to induce the survival of human neural stem cells in the absence of growth factors and to have therapeutic effects on neurodegenerative diseases. However, the molecular mechanisms of stemazole against apoptosis are ambiguous. In this study, tandem mass tag (TMT)-based proteomics was performed to obtain whole protein expression profiles of human neural stem cells in different groups, which was performed with or without stemazole and under extreme conditions. Bioinformatics analysis based on protein–protein interaction (PPI) network construction, gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis were adopted to explore crucial proteins and possible pharmaco-logical mechanisms. A total of 77 differentially expressed proteins were identified, comprising 38 upregulated proteins and 39 downregulated proteins. FN1(Fibronectin), Asparagine synthetase (ASNS), Phosphoserine aminotransferase (PSAT1), PKA C-alpha (PRKACA), Phospholipase C beta3 (PLCB

ORGANISM(S): Homo Sapiens

SUBMITTER: Mei Han  

PROVIDER: PXD032898 | iProX | Sat Apr 02 00:00:00 BST 2022

REPOSITORIES: iProX